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Leucocyte depletion does not affect post-ischaemic nerve cell damage in the rat

Acta Neurochirurgica volume 111, pages 54–60 (1991)Cite this article

Summary

Leucocytes play an important role in inflammation and immunologic responses. They might be of special significance under pathophysiological conditions of the brain i.e. ischaemia or stroke. It has been shown that neutropenic animals undergoing reversible ischaemia show higher post-ischaemic blood flow, suggesting improved post-ischaemic perfusion.

In this study it was investigated therefore, whether polymorphonuclear leucocytes contribute to the nerve cell loss in the hippocampus after a reversible period of ischaemia. Rats were made neutropenic with a specific anti-serum against rat polymorphonuclear leucocytes yielding leucocyte counts less than 10% of normal. The animals were then subjected to 15 min reversible forebrain ischaemia. Quantitative histology was performed after a survival period of 7 days. Nerve cell counts in the frontal cortex and in the CA1 and CA3 sectors of the hippocampus did not reveal any differences between neutropenic rats and animals with normal leucocyte counts.

From the results it might be concluded that neutrophils do not significantly contribute to the selective post-ischaemic nerve cell damage in the rat.

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References

  1. Ames A III, Wright RL, Kowada M, Thurston JM, Majano G (1968) Cerebral ischaemia. II. The no-reflow phenomenon. Am J Pathol 52: 437–453

    PubMed  Google Scholar 

  2. Aspey BS, Jessimer C, Pereira S, Harrison MJG (1989) Do leucocytes have a role in the cerebral no-reflow phenomenon? J Neurol Neurosurg Psychiatry 52: 526–528

    PubMed  Google Scholar 

  3. Beck Th, Nuglisch J, Sauer D, Bielenberg GW, Mennel HD, Rossberg C, Krieglstein J (1988) Effects of flunarizine on postischaemic blood flow, energy metabolism and neuronal damage in the rat brain. Europ J Pharmacol 158: 271–274

    Google Scholar 

  4. Bielenberg GW, Sauer D, Nuglisch J, Beck T, Rossberg C, Mennel HD, Krieglstein J (1989) Effects of emopamil on post-ischaemic blood flow and neuronal damage in rat brain. NaunynSchmiedeberg's Arch Pharmacol 339: 230–235

    Google Scholar 

  5. Church I, Zeman S, Lodge D (1988) The neuroprotective action of ketamine and MK-801 after transient cerebral ischaemia in rats. Anesthesiology 69: 702–709

    PubMed  Google Scholar 

  6. Deshpande JK, Wieloch T (1986) Flunarizine, a calcium entry blocker, ameliorates ischaemic brain damage in the rat. Anaesthesiology 64: 215–224

    Google Scholar 

  7. Ernst E, Matrai A, Paulsen F (1987) Leucocyte rheology in recent stroke. Stroke 18: 59–62

    PubMed  Google Scholar 

  8. Gaehtgens P, Ley K, Pries AR, Müller R (1985) Mutual interaction between leucocytes and microvascular blood flow. Progr Appl Microcirc 7: 15–28

    Google Scholar 

  9. Garcia JH, Lossinsky AS, Kauffman FC, Conger KA (1978) Neuronal ischaemic injury: Light microscopy, ultrastructure and biochemistry. Acta Neuropathol (Berl) 43: 85–95

    Google Scholar 

  10. Grögaard B, Schürer L, Gerdin B, Arfors K-E (1989) Delayed hypoperfusion after incomplete forebrain ischaemia in the rat. The role of polymorphonuclear leucocytes. J Cereb Blood Flow Metab 9: 500–505

    PubMed  Google Scholar 

  11. Grögaard B, Gerdin B, Reikeras O (1990) The polymorphonuclear leucocyte: has it a role in fracture healing? Arch Orthop Trauma Surg 109: 268–271

    PubMed  Google Scholar 

  12. Hallenbeck JM, Dutka AJ, Tanishima T, Kochanek PM, Kumaroo KK, Thompson CB, Obrenovitch TP, Contreras TJ (1986) Polymorphonuclear leucocyte accumulation in brain regions with low blood flow during the early post-ischaemic period. Stroke 17: 246–253

    PubMed  Google Scholar 

  13. Harlan IM (1985) Leucocyte-endothelial interactions. Blood 65: 513–525

    PubMed  Google Scholar 

  14. Holtz A, Nyström B, Gerdin B (1989) Spinal cord injury in rats: inability of nimodipine or anti-neutrophil serum to improve spinal cord blood flow or neurologic status. Acta Neurol Scand 79: 460–467

    PubMed  Google Scholar 

  15. Hossmann KA, Lechtape-Grüter H, Hossmann V (1973) The role of cerebral blood flow for the recovery of the brain after prolonged ischaemia. Z Neurol 204: 281–289

    PubMed  Google Scholar 

  16. Paschen W, Hossmann KA, van den Kerkhoff W (1983) Regional assessment of energy-producing metabolism following prolonged cerebral ischaemia of cat brain. J Cereb Blood Flow Metab 3: 321–329

    PubMed  Google Scholar 

  17. Pellegrino LJ, Pellegrino AS, Cushman AJ (1979) A stereotaxic atlas of the rat brain. Plenum Press, New York

    Google Scholar 

  18. Safar P (1986) Cerebral resuscitation after cardiac arrest. Circulation 74: 138–153

    Google Scholar 

  19. Sandler H, Högstorp H, Lundberg C, Gerdin B (1987) Antiserum-induced neutropenia in the rat: characterization of a rabbit anti-rat neutrophil serum. Br J Exp Pathol 68: 71–80

    PubMed  Google Scholar 

  20. Schmid-Schönbein GW (1987a) Leucocyte kinetics in the microcirculation. Biorheology 24: 139–151

    PubMed  Google Scholar 

  21. Schmid-Schönbein GW (1987b) Capillary plugging by granulocytes and the no-reflow phenomenon in the microcirculation. Fed Proc 46: 2397–2401

    PubMed  Google Scholar 

  22. Schmidt-Kastner R, Hossmann KA (1988) Distribution of ischaemic neuronal damage in the dorsal hippocampus of rat. Acta Neuropathol (Berl) 76: 411–421

    Google Scholar 

  23. Schmidt-Kastner R, Grosse Ophoff B, Hossmann KA (1990) Pattern of neuronal vulnerability in the cat hippocampus after one hour of global cerebral ischaemia. Acta Neuropathol (Berl) 79: 444–455

    Google Scholar 

  24. Schürer L, Grögaard B, Arfors K-E, Gerdin B (1988) Is postischaemic water accumulation related to delayed post-ischaemic hypoperfusion in rat brain? Acta Neurochir (Wien) 94: 150–154

    Google Scholar 

  25. Schürer L, Grögaard B, Gerdin B, Arfors K-E (1990) Superoxide dismutase does not prevent delayed hypoperfusion after incomplete cerebral ischaemia in the rat. Acta Neurochir (Wien) 103: 163–170

    Google Scholar 

  26. Smith M-L, Auer RN, Siesjö BK (1984) The density and distribution of ischaemic brain injury in the rat following 2–10 min of forebrain ischaemia. Acta Neuropathol (Berl) 64: 319–332

    Google Scholar 

  27. Snyder JV, Nemoto EM, Carroll RG, Safar P (1975) Global ischaemia in dogs: intracranial pressures, brain blood flow and metabolism. Stroke 6: 21–27

    PubMed  Google Scholar 

  28. Sutherland G, Ong BY, Louw D, Sima AAF (1989) Effect of lidocaine on forebrain ischaemia in rats. Stroke 20: 119–122

    PubMed  Google Scholar 

  29. Theodorsson-Norheim E (1986) Kruskal-Wallis test: BASIC computer program to perform non-parametric one-way analysis of variance and multiple comparisons on ranks of serveral independent samples. Comput Programs Biomed 23: 57–62

    Google Scholar 

  30. Warner DS, Godersky JC, Smith M-L (1988) Failure of preischaemic lidocaine administration to ameliorate global ischaemic brain damage in the rat. Anaesthesiology 68: 73–78

    Google Scholar 

  31. Wieloch T (1985) Neurochemical correlates to selective neuronal vulnerability. In: Kogure K, Hossmann KA, Siesjö BK, Welsh FA (eds) Progress in brain research, Vol 63. Elsevier Science Publishers, Amsterdam, pp 69–85

    Google Scholar 

  32. Yamakawa T, Yamaguchi S, Niimi H, Sugiyama I (1987) White blood cell plugging and blood flow maldistribution in the capillary network of cat cerebral cortex in acute haemorrhagic hypotension: an intravital microscopic study. Circ Shock 22: 323–332

    PubMed  Google Scholar 

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Author information

Affiliations

  1. Department of Experimental Medicine, Pharmacia AB, University Hospital, Uppsala, Sweden

    L. Schürer, B. Grögaard, B. Gerdin & K. -E. Arfors

  2. Department of Surgery, University Hospital, Uppsala, Sweden

    B. Gerdin

  3. Ulleval Hospital, Oslo, Norway

    B. Grögaard

  4. Institute for Surgical Research, University of Munich, Germany

    L. Schürer & O. Kempski

Authors
  1. L. Schürer
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  2. B. Grögaard
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  3. B. Gerdin
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  4. O. Kempski
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  5. K. -E. Arfors
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Schürer, L., Grögaard, B., Gerdin, B. et al. Leucocyte depletion does not affect post-ischaemic nerve cell damage in the rat. Acta neurochir 111, 54–60 (1991). https://doi.org/10.1007/BF01402514

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  • DOI: https://doi.org/10.1007/BF01402514

Keywords

  • Leucocytes
  • ischaemia
  • rat
  • selective vulnerability
  • quantitative histology