Summary
In 15 patients without acute brain injury the concentrations of Neuron-specific Enolase (NSE), S-100 Protein (S-100), Creatine Kinase (CK), and Creatine Kinase BB isoenzyme (CK-BB) in ventricular cerebrospinal fluid (CSF) were measured immediately after lateral ventricle cannulation for diagnostic or treatment purposes. From patients who were treated with a shunt another CSF sample was obtained one week after shunt implantation by puncture of the antechamber of the valve. The CSF concentrations of NSE, S-100, CK and CK-BB after cannulation were found to be of the same order as found in patients with severe head injury, stroke or subarachnoid haemorrhage. One week after shunt implantation the concentrations of S-100, CK and CK-BB had returned to normal levels in almost all patients, while the NSE concentrations remained elevated. These findings indicate that the sampling procedure may result in contamination of CSF with NSE, S-100, CK and CK-BB and they should be taken into account in the prognostic evaluation of enzyme concentrations after brain injury.
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Anagnostopoulos DI, Dontas IA, Kotsarelis DV, Julien G, Ka-rayannacos PE, Diakolios CE, Skalkeas G (1988) Creatine kinase (CK-BB) determination in cerebrospinal fluid after acute experimental head injury. Br J Neurosurg 2: 169–172
Armbruster DA, Gruemer H-D (1988) The regional variability of enzymes in the brain: relevance to CSF enzyme determinations. Clinica Chimica Acta 175: 227–238
Bach FW, Pedersen AG (1987) Creatine kinase B activity in cerebrospinal fluid. A marker of meningeal carcinomatosis in small cell lung cancer. In: Moss DW, Schmidt E, Schmidt FW (eds) Advances in clinical enzymology, Vol 5. Karger, Basel, pp 131–136
Bach FW, Kruse A, Melgaard B, Børgesen SE (1988) Creatine kinase BB release into cerebrospinal fluid after lateral ventricle cannulation. Br J Neurosurg 2: 339–42
Bakay R, Sweeney K, Wood J (1986) Pathophysiology of cerebrospinal fluid in head injury: Part 2. Biochemical markers for central nervous system trauma. Neurosurgery 18: 376–382
Calisanno P, Moore B, Friesen A (1969) Effect of calcium on S-100, a protein of the nervous system. Biochemistry 8: 4318–4326
Chandler WL, Clayson KJ, Longstreth WT jr, Fine JS (1984) Creatine kinase isoenzymes in human cerebrospinal fluid and brain. Clin Chem 30: 1804–1806
Chandler WL, Fine JS, Emery M, Weaver D, Reichenbach D, Clayson KJ (1988) Regional creatine kinase, adenylat kinase, and lactate dehydrogenase in normal canine brain. Stroke 19: 251–255
Endo E, Tanaka T, Kasai H, Okuyama T, Hidaka H (1981) Calcium dependent affinity chromatography of S-100 and calmodulin antagonist coupled sepharose. J Biol Chem 256: 12485–12489
Halonen T, Nantö V, Frey H, Lövgren T (1982) Bioluminescent assay of total and brain-specific creatine kinase activity in cerebrospinal fluid. J Neurochem 39: 36–43
Hans P, Albert A, Franssen C, Born J (1989) Improved outcome prediction based on CSF extrapolated creatine kinase BB isoenzyme activity and other risk factors in severe head injury. J Neurosurg 71: 54–58
Hårdemark H-G (1988) S-100 protein and neuron specific enolase cerebrospinal fluid markers of brain damage — clinical and experimental studies. Thesis. Uppsala university.
Jensen R, Marshak D, Anderson C (1985) Characterization of human brain S-100 protein fraction: Amino acid sequence of S-100beta. J Neurochem 45: 700–705
Kato K, Suzuki F, Semba R (1981) Determination of brain enolase isoenzymes with an enzyme immunoassay at the level of single neurons. J Neurochem 37: 998–1002
Marangos P, Schmeckel D, Parma A, Clark R, Goodwin F (1979) Measurement of neuron-specific enolase (NSE) and nonneuronal (NNE) isoenzymes of enolase in rat, monkey and human nervous tissue. J Neurochem 33: 319–329
Moore B (1972) Chemistry and biology of two proteins, S-100 and 14-3-2, specific to the nervous system. Int Rev Neurobiol 15: 215–223
Nordby HK, Urdal P (1982) The diagnostic value of measuring creatine kinase BB activity in cerebrospinal fluid following acute head injury. Acta Neurochir (Wien) 65: 93–101
Pasaoglu A, Pasaoglu H (1989) Enzymatic changes in the cerebrospinal fluid as indices of pathological change. Acta Neurochir (Wien) 97: 71–76
Persson L, Hårdemark H, Gustafsson J, Rundstrom G, Mendel-Hartvig I, Esscher T, Pøahlman S (1987) S-100 protein and neuron specific enolase in cerebrospinal fluid and serum: Markers of cell damage in human central nervous system. Stroke 18: 911–918
Rabow L, DeSalles AAF, Becker DP, Yang M, Kontos HA, Ward JD, Moulton RJ, Clifton G, Gruemer HD, Muizelaar P, Marmarou A (1986) CSF brain creatine kinase levels and lactic acidosis in severe head injury. J Neurosurg 65: 625–29
Rabow L, Cook D, Lipper MH, DeSalles AAF, Gruemer HD, Marmarou A, Becker DP (1989) Relationship between CT attenuation changes and post-traumatic CSF-CKBB-activity after severe head injury in man. Acta Neurochir (Wien) 100: 155–157
Thompson RJ, Kynoch PAM, Sarjant J (1980) Immunohistochemical localization of creatine kinase BB isoenzyme to astrocytes in human brain. Brain Res 201: 423–426
Yoshimine T, Morimoto K, Homburger HA (1983) Immunohistochemical localization of creatine kinase BB isoenzyme in human brain. Comparison with tubulin and astroprotein. Brain Res 265: 101–108
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Kruse, A., Cesarini, K.G., Bach, F.W. et al. Increases of Neuron-specific Enolase, S-100 Protein, Creatine Kinase and Creatine Kinase BB isoenzyme in CSF following intraventricular catheter implantation. Acta neurochir 110, 106–109 (1991). https://doi.org/10.1007/BF01400675
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DOI: https://doi.org/10.1007/BF01400675