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A structured model for the kinetics of fungal amylase production

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Summary

The cell is considered to be divided into nucleic acids, proteinaceous material and storage compounds. The enzyme is believed to be constitutive but repressed by the rate of catabolism. A structured model is developed to describe the growth, amylase production and dissolved oxygen profile in the batch culture ofAspergillus oryzae.

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Abbreviations

CA:

Conc. of enzyme SKB units/m3

CD,CE,CG,CO,CS :

Conc. of D.E.G. mass, oxygen, substrate kg/m3

CO*:

Mean oxygen conc. in gas-liquid interface kg/m3

CX :

Total cell conc. kg/m3

D:

D-mass (proteins) kg

E:

E-mass (storage carbon) kg

G:

G-mass (necleic acids) kg

KE :

Rate of usage of E-mass kg/m3/h

KEO :

KE with oxygen limitation kg/m3/h

Q:

Active fraction of promotor genes -

RD,RE,RG :

Rate of production of D,E,G-mass kg/m3/h

RO,RS :

Rate of usage of oxygen, substrate kg/m3/h

RDO,REO,RGO :

RD,RE and RG with oxygen limitation kg/m3/h

S:

Substrate (carbon) concentration kg/m3

t:

Time h

to :

Time at which CS = 0 h

K1,K2,K3,K24,K25 :

Stoichiometric constants -

K4,K5,K6,K7 :

Rate constants h−1

K8-K12,K18,K20-K23 :

Michaelis-Menten constants kg/m3

K26,K26 :

Absorption coefficient, K26 at CX = 0 h−1

K27 :

Empirical constant kg/m3

K15 :

Rate of enzyme formation SKB units/kg

K16,K17 :

Equilibrium constants m3/kg

K19 :

Decay constant for mRNA h−1

References

  • Caspersson, T. (1947)Symp. Soc. Exper. Biol. No. 1, Cambridge: University Press.

    Google Scholar 

  • Emmer, M., de Crombruggle, B., de Crombruggle, B., Pastan, I. and Perlman, R., (1970)Proc. Nat. Acad. Sci. 66, 480–487.

    PubMed  Google Scholar 

  • Enatsu, T. and Shinmyo, A. (1978)Adv. Biochem. Eng. 9, 111–144.

    Google Scholar 

  • Fitzpatrick, S.W. (1977), Ph.D. Thesis, U.M.I.S.T.

  • Gale, E. and Folkes, J.P. (1953)Biochem. J. 53, 483–492.

    PubMed  Google Scholar 

  • Herbert, D. (1958) inRecent progress in microbiology, G. Tunesall ed. 381–396 Oxford: Blackwell.

    Google Scholar 

  • Imanaka, T. and Aiba, S. (1977)Biotechnol. Bioeng. 19,757–764.

    PubMed  Google Scholar 

  • Inkson, M.B. (1972) Ph.D. Thesis, U.M.I.S.T.

  • Meyrath, J. and Volavsek, G. (1975) inEnzymes in food processing, 2nd Edition. G. Reed ed. 255–300, New York and London: Academic Press.

    Google Scholar 

  • Ramkrishna, D., Fredrickson, A.G. and Tsuchiya H.M. (1967)Biotechnol. Bioeng. 9, 129–170.

    Google Scholar 

  • Ryder, D.N. and Sinclair, C.G. (1972)Biotechnol. Bioeng. 14, 787–798.

    PubMed  Google Scholar 

  • Terui, G., Okazaki, M. and Kinochita, S. (1967),J.Ferment.Technol. 45, 497–503.

    Google Scholar 

  • van Dedem, G. and Moo-Young, M. (1973)Biotechnol, Bioeng. 15, 419–439.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Chemical Engineering, U.M.I.S.T., Manchester, England

    D. E. Brown & S. W. Fitzpatrick

Authors
  1. D. E. Brown
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  2. S. W. Fitzpatrick
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Brown, D.E., Fitzpatrick, S.W. A structured model for the kinetics of fungal amylase production. Biotechnol Lett 1, 3–8 (1979). https://doi.org/10.1007/BF01395782

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  • DOI: https://doi.org/10.1007/BF01395782

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