Summary
A technique is described for the separation and washing of soil micro-habitats (the separation being on a size basis). Tests of the efficiency of the washing technique show that, for the soil under experiment, 25 to 30 washings are required to remove most of the fungal spores; the number of washings required will vary from soil to soil. Data is given on the use of this method for fungal isolations from a cultivated soil.
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References
Chesters, C. G. C., A contribution to the study of fungi in the soil. Trans. Brit. Mycol. Soc.30, 100–117 (1948).
Glynne, M. D., Fungal invasion of roots of healthy wheat plants. Trans. Brit. Mycol. Soc.23, 210 (1939).
Harley, J. L. and Waid, J. S., A method of studying active mycelia on living roots and other surfaces in the soil. Trans. Brit. Mycol. Soc.38, 104–118 (1955).
Kurbis, W. P., Mykologische Untersuchungen über den Wurzelbereich der Ersche. Flora (Jena)131, 129–175 (1937).
Parkinson, D. and Kendrick, W. B., Investigations of soil microhabitats.In: Ecology of Soil Fungi. Pp. 22–28. Liverpool Univ. Press. (1960).
Robertson, N. F.: Studies on mycorrhizes of Pinus sylvestris. New Phytol.53, 253–283. (1954).
Simmonds, P. M., A washing device for isolation work with plant material. Phytopathology20, 911–913 (1930).
Warcup, J. H., Isolation of fungi from hyphae present in soil. Nature175, 953 (1955).
Warcup, J. H., Methods for isolation and estimation of activity of fungi in soil.In: Ecology of Soil Fungi. Pp. 3–21. Liverpool Univ. Press (1960)
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Parkinson, D., Williams, S.T. A method for isolating fungi from soil microhabitats. Plant Soil 13, 347–355 (1960). https://doi.org/10.1007/BF01394646
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DOI: https://doi.org/10.1007/BF01394646