The effect of D2O on the excitation properties of squid giant axons has been studied with the voltage-clamp technique. A decrease by a factor 1.4 in the amplitude of the maximum inward and outward currents was observed together with an increase, by about the same factor, in the time constants.
The result is in agreement with the known observation that D2O has no effect on the amplitude of the spike, since the currents measured during voltage clamp have a direct influence on the rate of change in the rising and falling phases, rather than on the amplitude of the action potential.
KeywordsTime Constant Nerve Fiber Environmental Physic Direct Influence Outward Current
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