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Simultaneous immunohistochemical demonstration of vasoactive intestinal polypeptide and its receptor in human colon

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Summary

The present study reports an immunohistochemical approach for localizing the immunoreactivity of vasoactive intestinal polypeptide (VIP) receptor in the human colon, by using a monoclonal antibody which recognizes the VIP-receptor of a human colonic adenocarcinoma cell line. Simultaneous demonstration of immunoreactive VIP-receptor of a human colonic adenocarcinoma cell line. Simultaneous demonstration of immunoreactive VIP-receptor and VIP was achieved by a double-labelling procedure employing immunogold silver staining for VIP-receptor, and a biotinylated secondary antibody followed by streptavidin-Texas Red, to visualize VIP.

The immunoreactive VIP receptor was found at two locations receiving dense VIP innervation: myenteric ganglia and mucosal epithelium.

Epithelial cells displayed intense labelling at the basolateral membrane, which confirmed earlier binding studies on fractionated membranes. A small number of enteroendocrine cells was also recognized by the VIP-receptor antibody. Smooth muscle and cells of the immune system were not stained by the monoclonal antibody, indicating that it recognized an epitope not common to VIP-receptors of all locations.

Thus, the immunohistochemical approach of VIP-receptor localization differs from autoradiography in (a) precise cellular localization, (b) possibility of simultaneous demonstration of receptor and ligand immunoreactivity, and (c) selectivity to a certain receptor population which, however, is presently not fully characterized.

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  1. Institute for Anatomy and Cell Biology, University of Heidelberg, Im Neuenheimer Feld 307, 6900, Heidelberg, Germany (FRG)

    W. Kummer

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Kummer, W. Simultaneous immunohistochemical demonstration of vasoactive intestinal polypeptide and its receptor in human colon. Histochem J 22, 249–256 (1990). https://doi.org/10.1007/BF01387180

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  • DOI: https://doi.org/10.1007/BF01387180

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