Summary
The production of penicillin amidase fromBacillus megaterium ATCC 14945 was examined in a sulfur-limited chemostat. Below 50% of the maximum growth rate, enzyme production was independent of dilution rate; above this point production decreased as growth rate increased. A simple and defined medium forBacillus licheniformis 749/C was developed, and extracellular penicillinase production was studied in a chemostat under sulfur- and nitrogenlimited conditions. While the yield of this enzyme differed little between these growth-limiting conditions, the specific activity of the penicillinase per unit weight of extracellular protein was increased 50% under nitrogenlimited conditions. Furthermore, the proportion of extracellular enzyme was greater in continuous, than in batch culture.
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Acevedo, F., Cooney, C.L. Penicillin amidase and penicillinase production in nitrogen- and sulfur-limited chemostats. European J. Appl Microbiol. 2, 9–17 (1975). https://doi.org/10.1007/BF01385441
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DOI: https://doi.org/10.1007/BF01385441