Summary
Rod-shaped inclusions in the nuclei and/or cytoplasm of strains ofEuglena spirogyra, Lepocinclis ovum, Strombomonas conspersa andTrachelomonas oblonga var.punctata have been identified as living populations of bacteria by reference to size, shape and ultrastructure. Most strains contain 20–200 bacteria per nucleus. Each bacterial cell is surrounded by a mucilaginous sheath and consists of a double-membraned envelope, dense cytoplasm with small ribosomes, and a filamentous DNA-containing centre. The bacteria multiply to keep pace with euglenoid division but there is no evidence that the euglenoid cells benefit from or are harmed by the association. Attempts to transfer the endonuclear bacteria to “uninfected” strains and species have been unsuccessful, as have attempts to isolate the bacterium into pure culture. The “infected” strain ofEuglena spirogyra cannot fix nitrogen. The possible nature of the euglenoid/bacterium association is discussed.
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References
Burris, R. H., andP. W. Wilson: Methods for measurement of nitrogen fixation. Methods Enzymol.4, 355–366 (1957).
Dangeard, P. A.: Nouvelles observations sur les parasites des Eugleniens. Botaniste25, 3 (1933).
Fogg, G. E.: Nitrogen fixation by photosynthetic organisms. Ann. Rev. Plant. Physiol.7, 52–70 (1956).
Leedale, G. F.: Nuclear structure and mitosis in the Euglenineae. Arch. Mikrobiol.32, 32–64 (1958).
—: Endonuclear bacteria in euglenoid flagellates J. Protozool.13 (Suppl.), 22 (1966).
—: Endonuclear bacteria in species ofEuglena, Strombomonas andTrachelomonas. Brit. Phycol. Bull.3, 413 (1967 a).
—: Euglenoid Flagellates. Englewood Cliffs: Prentice-Hall 1967 (1967 b).
—: The nucleus inEuglena, in:Buetow, D. E. (Ed.): The Biology ofEuglena. New York and London: Academic Press 1968.
Leedale, G. F., B. J. D. Meeuse andE. G. Pringsheim: Structure and physiology ofEuglena spirogyra. Arch. Mikrobiol.50, 68–102, 133–155 (1965).
Pringsheim, E. G.: The biphasic or soil-water culture method for growing algae and flagellata. J. Ecol.33, 193–204 (1946).
Reynolds, S.: The use of lead citrate at highPh as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–211 (1963).
Roth, L. E.: An electron-microscope study of the cytology of the protozoanPeranema trichophorum. J. Protozool.6, 107–116 (1959).
Sagan, L.: On the origin of mitosing cells. J. Theoret. Biol.14, 225–274 (1967).
Ueda, K.: Structure of plant cells with special reference to lower plants IV. Structure ofTrachelomonas sp. Cytologia25, 8–16 (1960).
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Dedicated to Prof. Dr.Lothar Geitler on the occasion of his 70th birthday.
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Leedale, G.F. Observations on endonuclear bacteria in euglenoid flagellates. Österr bot Z 116, 279–294 (1969). https://doi.org/10.1007/BF01379628
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DOI: https://doi.org/10.1007/BF01379628