Summary
Addition of colchicin to nutrient solutions supplied to red clover plants had an inhibitory effect on root development and nodulation. At the lowest colchicin concentrations, a small, non-significant increase in the number of nodules was often observed; in a few experiments a barely significant increase in the number of nodules developing was observed up to concentrations of 10 mg per 1.
Colchicin did not influence the growth of Rhizobium and growth of the bacteria in the presence of colchicin (10 mg per 1) had no effect on their subsequent infectivity or ability to fix nitrogen.
Treatment with colchicin resulted in the formation of increased numbers of polyploid cells of the clover roots. In addition, it induced the development of deformed root-hairs which probably facilitated infection by Rhizobium and the formation of infection threads.
The nodules developing at colchicin concentrations of 10 mg per 1 and higher were small, had an abnormal structure and were ineffective in nitrogen fixation.
It is concluded that any stimulatory effect of colchicin on nodulation does not necessarily require to be associated with an increase in the number of polyploid cells in the root cortex. The effect of colchicin can be fully accounted for, initially by the greater number of infections resulting from the development of increased numbers of deformed root hairs, and subsequently by the reduced inhibitory action of the ineffective nodules on the formation of further nodules.
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Literature
Allen, M. B. and Arnon, D. I., Studies on nitrogen-fixing blue-green algae I. Growth and nitrogen fixation byAnabaena cylindrica Lem. Plant Physiol.30, 366–372 (1955).
Bhaskaran, S. and Swaminathan, M. S., Polyploidy and the genesis of the leguminous root nodules. The Nucleus1, 75–88 (1954).
Bonnier, G., Action de la colchicine sur la symbioseRhizobium-Medicago sativa etRhizobium-Trifolium pratense. Bull Inst. Agron. Sta. Rech. Gembloux22, 167–178 (1954).
Darlington, D. C. and La Cour, I. F., The Handling of Chromosomes. London (1950).
Davidson, D. A., A different response to colchicin of meristems of roots ofVicia faba. Ann. Botany N.S.29, 253–264 (1965).
Davidson, D., Macleod, R. D. and Taylor, J., An IAA-induced stimulation of growth of roots ofVicia faba previously treated with colchicin. New Phytologist64, 393–398 (1965).
Deeley, E. M., Davies, H. G. and Chayen, J., The DNA content of cells in the root ofVicia faba. Exp. Cell Research12, 852–591 (1957).
Fahraeus, G., The infection of clover root hairs by nodule bacteria studied by a simple glass slide technique. J. Gen. Microbiol.16, 374–381 (1957).
Fred, E. B., Baldwin, I. L., MacCoy, E., Root nodule bacteria and leguminous plants. Univ. Wisconsin Studies5, Madison (1932).
Macleish, J. and Sunderland, N., Measurements of desoxyribose-nucleic acid (DNA) in higher plants by Feulgen photometry and chemical methods. Exp. Cell Research24, 527–540 (1961).
Mitchell, J. P., The DNA content of nuclei in pea root nodules. Ann. Botany N.S.29, 371–376 (1965).
Nutman, P. S., Physiological studies on nodule formation. The relation between nodulation and lateral root formation in red clover. Ann. Botany N.S.12, 81–96 (1948).
Nutman, P. S., Studies on the physiology of nodule formation. Experiments on the excision of root tips and nodules. Ann. Botany N.S.16, 79–101 (1952).
Nutman, P. S., Some observations on root hairs infection by nodule bacteria. J. Exp. Botany10, 250–263 (1959).
Paech, K., and Tracey, M. V., Moderne Methoden der PflanzenanalyseIV, 435 (1955).
Papaioannou, C., Contribution a l'étude de l'action des substances mutagènes: isosulfocyanate d'allyle et colchicine sur le metabolisme auxinique et conséquences génétiques. Ann. Inst. Expt. Tabac Bergerac3, 511–554 (1960).
Sahlman, K. G. and Fåhraeus, G., Microscopic observations on the effect of indole-3-acetic acid upon root hairs of Trifolium repens. Kungl. Lantbrukshögsk. Ann.28, 261–268 (1962).
Schiel, E., Olivero, E. L. G. de, Dieguez, R. N., Pacheco, J. C. and Enokida, E., Effect de la colchicine sur la virulence et l'efficacité de Rhizobium melilote sur la luzerne. Ann. Inst. Pasteur105, 332–340 (1963).
Trolldenier, G., Polyploidie und Knöllchenbildung bei Leguminosen. thesis Fakult. Landbautech. Univ. Berlin. nr.71, (1958).
Trolldenier, G., Polyploidie und Knöllchenbildung bei Leguminosen. Arch. Mikrobiol.32, 328–345 (1959).
Wabeke, D. and van Eeden, C., Handleiding voor de Toets van Wilcoxon. Amsterdam Mathematisch Centrum (1955).
Weir, J. B., The effect of colchicine and indolylacetic acid on diploid and tetraploid strains of red and white clovers in aseptic and pot cultures. Plant and Soil14, 187–196 (1961).
Wipf, L. and Cooper, C., Chromosome numbers in root nodules and roots of red clover, common vetch and garden pea. Proc. Nat. Acad. Sci. U.S.A.24, 87–91 (1938).
Wipf, L. and Cooper, D. C., Somatic doubling of chromosomes and nodular infection in certain Leguminosae. Am. J. Botany27, 821–824 (1940).
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Van Der Starre-Van Der Molen, L.G., Bossink, G.A.H. & Quispel, A. The influence of colchicin on root nodule formation inTrifolium Pratense . Plant Soil 26, 397–412 (1967). https://doi.org/10.1007/BF01379562
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DOI: https://doi.org/10.1007/BF01379562