Summary
The distribution of actin on various organelles in a green alga,Trebouxia potteri, was examined by immunoelectron microscopy. Actin was detected on the surface of lysosomes at various stages during the formation of zoospores. The distribution of actin on the surface of lysosomes is discussed in connection with their change in shape at a specific stage during the formation of zoospores. Actin was also detected on the surface of coated vesicles, Golgi vesicles, and the trans Golgi network, while it was not detected on the surfaces of mitochondria, chloroplasts, and Golgi bodies.
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Abbreviations
- BSA:
-
bovine serum albumin
- ER:
-
endoplasmic reticulum
- PBS:
-
phosphate buffered saline
- TGN:
-
trans Golgi network
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Chida, Y., Ueda, K. Detection of actin on organelles ofTrebouxia potteri, in particular on the surface of lysosomes. Protoplasma 171, 28–33 (1992). https://doi.org/10.1007/BF01379277
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DOI: https://doi.org/10.1007/BF01379277