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Inter- and intra-strain genomic variation in equine herpesvirus type 1 isolates

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Summary

Restriction enzyme digests of DNA from 22 unselected isolates of EHV-1 were analysed by hybridization with cloned DNA fragments covering the genome. In addition to a small amount of inter-strain variation, heterogeneity within strains was observed, caused by loss of specific restriction endonuclease sites in the DNA of a proportion of the virus particles of any one stock. Fifteen strains demonstrated the same intra-strain variation involving loss of the BamHI L-M site which was shown to lie within coding sequence for the large subunit of ribonucleotide reductase. This particular mutation may therefore be selected for by passage in RK13 cells.

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Author notes

  1. W. A. Bonass

    Present address: Division of Oral Biology, Leeds Dental Institute, University of Leeds, Leeds, UK

  2. D. M. Elton

    Present address: Department of Entomology, Texas A&M University, College Station, Texas, USA

Authors and Affiliations

  1. Department of Microbiology, University of Leeds, Leeds, UK

    W. A. Bonass, W. A. Hudson, D. M. Elton, R. A. Killington & I. W. Halliburton

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  1. W. A. Bonass
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  2. W. A. Hudson
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  3. D. M. Elton
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  4. R. A. Killington
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  5. I. W. Halliburton
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Bonass, W.A., Hudson, W.A., Elton, D.M. et al. Inter- and intra-strain genomic variation in equine herpesvirus type 1 isolates. Archives of Virology 134, 169–178 (1994). https://doi.org/10.1007/BF01379115

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  • DOI: https://doi.org/10.1007/BF01379115

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