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Characterization of the transforming domain of a feline sarcoma virus encoding afgr-related tyrosine kinase

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Summary

We investigated the transforming domain of a recently isolated feline sarcoma virus (TP1-FeSV) which encodes afgr-related tyrosine kinase expressed as agag-fgr fusion protein. Thegag portion was removed and replication-competent expression vectors (RCAS) with insertedv-fgr sequences were established. Chicken embryo fibroblasts (CEF) were transfected and monitored for replication, integration and transcription of the proviral constructs. We demonstrated that transfected cells display morphological changes and are able to form colonies in soft-agar. This suggests that thegag portion of the fusion protein from TP1-FeSV is not necessary for the transformation of fibroblasts.

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  1. Department of Experimental Tumorbiology, University of Muenster, Muenster, Federal Republic of Germany

    G. Weyrauch & Angelika Barnekow

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  1. G. Weyrauch
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  2. Angelika Barnekow
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Weyrauch, G., Barnekow, A. Characterization of the transforming domain of a feline sarcoma virus encoding afgr-related tyrosine kinase. Archives of Virology 134, 141–155 (1994). https://doi.org/10.1007/BF01379113

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  • DOI: https://doi.org/10.1007/BF01379113

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