Summary
The frequency of nonhemagglutinating (h−) mutants was determined in hemagglutinating (HA) clones of echovirus 11. The HA clones were derived from the prototype strain Gregory and a clinical isolate of echovirus 11. The h− mutants were found at a constant frequency of 3.0×10−5 (mean value) in the HA clones derived from both strains. Since the conditions of the population equilibrium of HA clones and neutrality of the mutation were satisfied, it was proposed that the observed frequency of h− mutants occurred as a result of a point mutation. The frequency of h+ revertants at the second passage of h− mutant clone was 9.1×10−5. Hence the frequency of reversion suggests that the number of potential sites of back mutation was restricted.
Similar content being viewed by others
References
Auvinen P, Hyypiä T (1990) Echoviruses include genetically distinct serotypes. J Gen Virol 71: 2133–2139
Cova L, Aymard M (1980) Isolation and characterization of non-hemagglutinating echovirus 11. J Gen Virol 51: 219–222
De la Torre JC, Wimmer E, Holland JJ (1990) Very high frequency of reversion to guanidine resistance in clonal pools of guanidine-dependent type 1 poliovirus. J Virol 64: 664–671
Domingo E, Holland JJ (1988) High error rates, population equilibrium and evolution of RNA replication systems. In: Domingo E, Holland JJ, Ahlquist P (eds) RNA genetics, Vol 3. CRC Press, Boca Raton, pp 3–36
Dulbecco R, Vogt M (1954) Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med 99: 167–182
Eigen M, Biebricher CK (1988) Sequence space and quasi-species distribution. In: Domingo E, Holland JJ, Ahlquist P (eds) RNA genetics, Vol 3. CRC Press, Boca Raton, pp 211–245
Goldfield M, Srihongse, Fox JP (1957) Hemagglutinins associated with certain human enteric viruses. Proc Soc Exp Biol Med 96: 788–791
Heinz BA, Rueckert RR, Shepard DA, Dutko FJ, McKinlay MA, Fancher M, Rossmann MG, Badger J, Smith TJ (1989) Genetic and molecular analyses of spontaneous mutants of human rhinovirus 14 that are resistant to an antiviral compound. J Virol 63: 2476–2485
Mahy BWJ (ed) (1985) Virology. A practical approach. IRL Press, Oxford
Maurseth A, Flugsrud L, Lahelle O (1960) Variation of hemagglutination within ECHO 6 strains. Acta Pathol Microbiol Scand 50: 444
Mutanda LN, Mufson MA (1974) Characterization of echovirus 7, 12, and 29 hemagglutinin for human and monkey erythrocytes. Proc Soc Exp Biol Med 145: 1008–1013
Philipson L, Bengtsson (1962) Interaction of enteroviruses with receptors from erythrocytes and host cells. Virology 18: 457–469
Philipson L, Lind M (1964) Enterovirus eclipse in a cell-free system. Virology 23: 322–332
Podoplekin VD (1964) Effect of tissue cultures on the hemagglutinating properties of echoviruses and mechanism of the interaction of these viruses with erythrocytes. Acta Virol (Praha) 8: 254–262
Simon M, Dömök I (1963) Studies on intratypic variants of echoviruses. I. Separation of haemagglutinating and non-haemagglutinating virus particles from echovirus type 6. Acta Microbiol Acad Sci Hung 10: 293–306
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sergeev, A.G., Novoselov, A.V., Bubenschikov, A.V. et al. Genetic analysis of echovirus 11 variability in adsorption to human erythrocytes. Archives of Virology 134, 129–139 (1994). https://doi.org/10.1007/BF01379112
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01379112