Skip to main content
SpringerLink
Log in

Lectin-mediated enhancement of dengue virus infection in a mouse macrophage cell line Mk1

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Treatment of a mouse macrophage cell line Mk1 with pokeweed mitogen (PWM) either before or during but not after virus inoculation resulted in an enhancement of dengue virus (DV) infection. The infection enhancement was primarily due to an increase in the number of DV-infected cells but not to increased virus production in a cell. These results suggested that PWM treatment mediated increased DV binding and/or penetration to Mk1 cells, thereby resulting in the infection enhancement.N-acetylglucosamine (GlucNAc) did not suppress PWM-mediated enhancement of DV infection when added to Mk1 cells after PWM treatment was done, although GlucNAc clearly suppressed the effect of PWM when added simultaneously with PWM. The results implied the possibility that the PWM-mediated increase in viral binding/penetration was not due to a cross-linking by PWM between DV and a cell-surface receptor, but due to another mechanism, presumably exposure of a masked DV receptor(s). The DV receptor, unidentified as yet, involved in the PWM-mediated infection enhancement appeared to have no relation with IgG Fc receptors that are known to be involved in antibody-mediated enhancement of DV infection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Daughaday CC, Brandt WE, McCown JM, Russell PK (1981) Evidence for two mechanisms of dengue virus infection of adherent human monocytes: trypsin-sensitive virus receptors and trypsin-resistant immune complex receptors. Infect Immun 32: 469–473

    PubMed  Google Scholar 

  2. Esumi-Kurisu M, Okutomi T, Mizuno D, Yamazaki M (1983) Comparative studies on antibody and lectin-dependent macrophage-mediated tumor lysis. J Reticuloendothel Soc 33: 83–92

    PubMed  Google Scholar 

  3. Finkelstein MS, McWilliams M (1976) Effects of plant lectins on viral growth in nonlymphoid cells. Virology 69: 570–586

    PubMed  Google Scholar 

  4. Gentry MK, Henchal EA, McCown JM, Brandt WE, Dalrymple JM (1982) Identification of distinct antigenic determinants on dengue-2 virus using monoclonal antibodies. Am J Trop Med Hyg 31: 548–555

    PubMed  Google Scholar 

  5. Halstead SB, O'Rourke EJ (1977) Dengue viruses and mononuclear phagocytes. I. Infection enhancement by non-neutralizing antibody. J. Exp Med 146: 201–217

    PubMed  Google Scholar 

  6. Halstead SB, O'Rourke EJ, Allison AC (1977) Dengue viruses and mononuclear phagocytes. II. Identity of blood and tissue leukocytes supportingin vitro infection. J Exp Med 146: 218–229

    PubMed  Google Scholar 

  7. Henchal EA, Gentry MK, McCown JM, Brandt WE (1982) Dengue virus-specific and flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am J Trop Med Hyg 31: 830–836

    PubMed  Google Scholar 

  8. Hotta H, Hotta S (1982) Dengue virus multiplication in cultures of mouse peritoneal macrophages: effects of macrophage activators. Microbiol Immunol 26: 665–676

    PubMed  Google Scholar 

  9. Hotta H, Hotta S, Homma M (1984) Effect of interferons on dengue virus multiplication in cultured monocytes/macrophages. Biken J 27: 189–193

    PubMed  Google Scholar 

  10. Hotta H, Hotta S, Takada H, Kotani S, Tanaka S, Ohki M (1983) Enhancement of dengue virus type 2 replication in mouse macrophage cultures by bacterial cell walls, peptidoglycans, and a polymer of peptidoglycan subunits. Infect Immun 41: 462–469

    PubMed  Google Scholar 

  11. Hotta H, Sanchez LF, Takada H, Homma M, Kotani S (1985) Enhancement of dengue virus infection in cultured mouse macrophages by lipophilic derivatives of muramyl peptides. Microbiol Immunol 29: 533–541

    PubMed  Google Scholar 

  12. Hotta H, Wiharta AS, Hotta S (1984) Antibody-mediated enhancement of dengue virus infection in mouse macrophage cell lines, Mk1 and Mm1. Proc Soc Exp Biol Med 175: 320–327

    PubMed  Google Scholar 

  13. Hotta H, Wiharta AS, Hotta S, Homma M (1984) Dengue type 2 virus infection in human peripheral blood monocyte cultures. Microbiol Immunol 28: 1099–1109

    PubMed  Google Scholar 

  14. Kontny U, Kurane I, Ennis FA (1988) Gamma interferon augments Fcγ receptor-mediated dengue virus infection of human monocytic cells. J Virol 62: 3928–3933

    PubMed  Google Scholar 

  15. Littaua, R, Kurane I, Ennis FA (1990) Human IgG Fc receptor II mediates antibody-dependent enhancement of dengue virus infection. J Immunol 144: 3183–3186

    PubMed  Google Scholar 

  16. Mady BJ, Erbe DV, Kurane I, Fanger MW, Ennis FA (1991) Antibody-dependent enhancement of dengue virus infection mediated by bispecific antibodies against cell surface molecules other than Fcγ receptors. J Immunol 147: 3139–3144

    PubMed  Google Scholar 

  17. Marchette NJ, Halstead SB (1978) Phytohemagglutinin enhancement of dengue-2 virus replication in nonimmune rhesus monkey peripheral blood leukocytes. Infect Immun 19: 40–45

    PubMed  Google Scholar 

  18. Polin RA, Kennett R (1980) Pokeweed mitogen inhibition of protein synthesis in cultured lymphoblastoid lines. In Vitro 16: 575–580

    PubMed  Google Scholar 

  19. Stevenson HC, Miller PJ, Waxdal MJ, Haynes BF, Thomas CA, Fauci AS (1983) Interaction of pokeweed mitogen with monocytes in the activation of human lymphocytes. Immunology 49: 633–640

    PubMed  Google Scholar 

  20. Sullivan JL, Barry DW, Lucas SJ, Albrecht P (1975) Measles infection of human mononuclear cells. I. Acute infection of peripheral blood lymphocytes and monocytes. J Exp Med 142: 773–784

    PubMed  Google Scholar 

  21. Teltow GJ, Irvin JD, Aron GM (1983) Inhibition of herpes simplex virus DNA synthesis by pokeweed antiviral protein. Antimicrob Agents Chemother 23: 390–396

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Kobe University School of Medicine, Chuo-ku, Kobe, Hyogo, Japan

    H. Hotta & M. Homma

Authors
  1. H. Hotta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Homma
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hotta, H., Homma, M. Lectin-mediated enhancement of dengue virus infection in a mouse macrophage cell line Mk1. Archives of Virology 134, 51–59 (1994). https://doi.org/10.1007/BF01379106

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01379106

Keywords

Search

Navigation