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The identification, cloning, and sequence analysis of the coat protein coding region of a birch isolate (I2) of cherry leaf roll nepovirus

Archives of Virology volume 131, pages 209–215 (1993)Cite this article

Summary

The coat protein gene of RNA-2 of cherry leaf roll nepovirus (CLRV) birch isolate I2 was cloned, identified, and sequenced. Transcripts derived from cDNA to the coat protein gene made a polypeptide of Mr 51.5 k when translated in vitro. The predicted amino acid sequence of the coat protein showed little identity with nepoviruses having small RNA-2s. It did, however, have 27% sequence identity with the coat protein of tomato ringspot nepovirus which, like CLRV, has a relatively large RNA-2.

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Author notes

  1. N. W. Scott

    Present address: Department of Applied Biology, and Biotechnology, DeMontfort University, Leicester, U.K.

Affiliations

  1. Institute of Virology and Environmental Microbiology, Oxford, U.K.

    N. W. Scott, J. I. Cooper & M. L. Edwards

Authors
  1. N. W. Scott
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  2. J. I. Cooper
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  3. M. L. Edwards
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Scott, N.W., Cooper, J.I. & Edwards, M.L. The identification, cloning, and sequence analysis of the coat protein coding region of a birch isolate (I2) of cherry leaf roll nepovirus. Archives of Virology 131, 209–215 (1993). https://doi.org/10.1007/BF01379093

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  • DOI: https://doi.org/10.1007/BF01379093

Keywords

  • Infectious Disease
  • Amino Acid Sequence
  • Sequence Analysis
  • Polypeptide
  • Protein Gene