Summary
The nucleotide sequence of the coat protein genes and 3′ non-coding regions of two different resistance-breaking tobamoviruses in pepper have been determined. The deduced coat protein of an Italian isolate of pepper mild mottle virus (PMMV-I) consists of 156 amino acids and its 3′ non-coding region is 198 nucleotides long. They have been found to be very similar in sequence and structure to those previously reported for a Spanish isolate (PMMV-S). In contrast, a Dutch isolate termed P 11 codes for a coat protein of 160 amino acids and its 3′ non-coding region is 291 nucleotides long, which may have arisen by duplication. The nucleotide and the predicted coat protein amino acid sequence analysis show that this isolate should be considered as a new virus within the tobamovirus group. The term paprika mild mottle virus (PaMMV) is proposed.
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Abrahams JP, Van der Berg M, Van Batenburg E, Pleij C (1990) Prediction of RNA secondary structure, including pseudoknotting, by computer simulation. Nucleic Acids Res 18: 3035–3044
Allison RF, Janda M, Ahlquist P (1989) Sequence of cowpea chlorotic mottle virus RNAs 2 and 3 and evidence of a recombination event during bromovirus evolution. Virology 172: 321–330
Alonso E, García-Luque I, Avila-Rincón MJ, Wicke B, Serra MT, Díaz-Ruíz JR (1989) A tobamovirus causing heavy losses in protected pepper crops in Spain. J Phytopathol 125: 67–76
Alonso E, García-Luque I, de la Cruz A, Wicke B, Avila-Rincón MJ, Serra MT, Castresana C, Díaz-Ruíz JR (1991) Nucleotide sequence of the genomic RNA of pepper mild mottle virus, a resistance-breaking tobamovirus in pepper. J Gen Virol 72: 2875–2884
Altschuh D, Lesk AM, Bloomer AC, Klug A (1987) Correlation of co-ordinated amino acid substitutions with function in viruses related to tobacco mosaic virus. J Mol Biol 193: 693–707
Avila-Rincón MJ, Ferrero ML, Alonso E, García-Luque I, Díaz-Ruíz JR (1989) Nucleotide sequences of 5′ and 3′ non-coding regions of pepper mild mottle virus strain S RNA. J Gen Virol 70: 3025–3031
Betti L, Tanzi M, Canova A (1986) Evolutionary changes in TMV pepper strains as a result of repeated host passages. Phytopathol Medit 25: 39–43
Boukema IW, Jansen K, Hofman K (1980) Strains of TMV and genes for resistance inCapsicum. In: Synopses of the fourth meeting of Eucarpia Capsicum Working Group, Wageningen 1980. Eucarpia 80, Wageningen, pp 44–48
Bujarski JJ, Kaesberg P (1986) Genetic recombination between RNA components of a multipartite plant virus. Nature 321: 528–531
Bujarski JJ, Ahlquist P, Hall TC, Dreher TW, Kaesberg P (1986) Modulation of replication, aminoacylation and adenylation in vitro and infectivity in vivo of BMV RNAs containing deletions within the multifunctional 3′ end. EMBO J 5: 1769–1774
Calder VL, Palukaitis P (1992) Nucleotide sequence analysis of the movement genes of resistance breaking strains of tomato mosaic virus. J Gen Virol 73: 165–168
Cascone PJ, Carpenter CD, Hua Li X, Simon AE (1990) Recombination between satellite RNAs of turnip crinkle virus. EMBO J 9: 1709–1715
Creaser EH, Gibbs AJ, Pares RD (1987) The amino acid composition of the coat protein of a tobamovirus from an AustralianCapsicum crop. Aust Plant Pathol 16: 85–87
Csillery G, Tóbiás I, Ruskó J (1983) A new pepper strain of tomato mosaic virus. Acta Phytopathol Acad Sci Hung 18: 195–200
Culver JN, Dawson WO (1989) Point mutations in the coat protein gene of tobacco mosaic virus induce hypersensitivity inNicotiana sylvestris. Mol Plant Microbe Interact 2: 209–213
Dreher TW, Bujarski JJ, Hall TC (1984) Mutant viral RNAs synthesized in vitro show altered aminoacylation and replicase template activities. Nature 311: 171–175
Feldman JM, Oremianer S (1972) An unusual strain of tobacco mosaic virus from pepper. Phytopathology 75: 250–267
Fraile A, García-Arenal F (1990) A classification of the tobamoviruses based on comparisons among their 126 K proteins. J Gen Virol 71: 2223–2228
García-Arenal F (1988) Sequence and structure at the genome 3′end of the U2-strain of tobacco mosaic virus, a histidine-accepting tobamovirus. Virology 167: 201–206
García-Luque I, Serra MT, Alonso E, Wicke B, Ferrero ML, Díaz-Ruíz JR (1990) Characterization of a Spanish strain of pepper mild mottle virus (PMMV-S) and its relationship to other tobamoviruses. J Phytopathol 129: 1–8
Gibbs A (1986) Tobamovirus classification. In: van Regenmortel MHV, Fraenkel-Conrat H (eds) The plant viruses, vol 2. Plenum, New York, pp 168–178
Goelet P, Lomonossoff GP, Butler PJG, Akam ME, Gait MJ, Karn J (1982) Nucleotide sequence of tobacco mosaic virus RNA. Proc Natl Acad Sci USA 79: 5818–5822
Gracia O, Feldman JM, Pontis RE, Boninsegna J (1968) Some viruses affecting tomatoes and peppers in Argentina. Plant Dis Rep 52: 674–676
Greenleaf WH, Cook AA, Heyn ANJ (1964) Resistance to tobacco mosaic virus in Capsicum, with reference to the Sansum latent strain. Phytopathology 54: 1367–1371
Gübler U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269
Hamilton WDO, Boccara M, Robinson DJ, Baulcombe DC (1987) The complete nucleotide sequence of tobacco rattle virus RNA-1. J Gen Virol 68: 2563–2575
Higgins DG, Sharp PM (1989) Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5: 151–153
Higgins DG, Bleasby AJ, Fuchs R (1992) Clustal V: improved software for multiple sequence alignments. CABIOS 8: 189–191
Isomura Y, Matumoto Y, Murayama A, Chatani M, Inouye N, Ikegami M (1991) Molecular cloning, sequencing and expression inEscherichia coli of the odontoglossum ringspot virus coat protein gene. J Gen Virol 72: 2247–2249
Keese P, Symons RH (1985) Domains in viroids: evidence of intermolecular RNA rearrangements and their contribution to viroid evolution. Proc Natl Acad Sci USA 82: 4582–4586
Kirkegaard K, Baltimore D (1986) The mechanism of RNA recombination in poliovirus. Cell 47: 433–443
Knorr DA, Dawson WO (1988) A point mutation in the tobacco mosaic virus capsid protein gene induces hypersensitivity inNicotiana sylvestris. Proc Natl Acad Sci USA 85: 170–174
Makino S, Keck JG, Stohlman SA, Lai MMC (1986) High-frequency RNA recombination of murine coronaviruses. J Virol 57: 729–737
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor
Marsh LE, Pogue GP, Hall TC (1989) Similarities among plant viruses (+) and (−) RNA termini imply a common ancestry with promoters of eukaryotic tRNAs. Virology 172: 415–427
Marte M, Wetter C (1986) Occurrence of pepper mild mottle virus in pepper cultivars from Italy and Spain. Z Pflanzenkrankh Pflanzenschutz 93: 37–43
Martelli GP, Quacquarelly A (1982) The present status of tomato and pepper viruses. Acta Hortic 127: 39–95
Maxam AM, Gilbert W (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol 65: 499–560
Mckinney HH (1952) Two strains of tobacco mosaic virus, one of which is seed-borne in an etch immune pungent pepper. Plant Dis Rep 36: 184–187
Meshi T, Ohno T, Iba H, Okada Y (1981) Nucleotide sequence of a cloned cDNA copy of TMV (cowpea strain) RNA, including the assembly origin, the coat protein cistron, and the 3′ non-coding region. Mol Gen Genet 184: 20–25
Meshi T, Kiyama R, Ohno T, Okada Y (1983) Nucleotide sequence of the coat protein cistron and the 3′ noncoding region of cucumber green mottle mosaic virus (watermelon strain) RNA. Virology 127: 54–64
Meshi T, Motoyoshi F, Adachi A, Watanabe Y, Takamatsu N, Okada Y (1988) Two concomitant base substitutions in the putative replicase genes of tobacco mosaic virus confer the ability to overcome the effects of a tomato resistance gene,Tm-1. EMBO J 7: 1575–1581
Meshi T, Motoyoshi F, Maeda T, Yoshiwoka S, Watanabe H, Okada Y (1989) Mutations in the tobacco mosaic virus 30-kD protein gene overcome Tm-2 resistance in tomato. Plant Cell 1: 515–522
Nejidat A, Cellier F, Holt CA, Gafny R, Eggenberger AL, Beachy RN (1991) Transfer of the movement protein gene between two tobamoviruses: influence on local lesion development. Virology 180: 318–326
Ohno T, Aoyagi M, Yamanashi Y, Saito H, Ikawa S, Meshi T, Okada Y (1984) Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome. J Biochem 96: 1915–1923
Pares RD (1985) A tobamovirus infecting capsicum in Australia. Ann Appl Biol 106: 469–474
Pares RD (1988) Serological comparison of an Australian isolate of Capsicum mosaic virus with capsicum tobamovirus. Ann Appl Biol 112: 609–612
Peattie DA (1979) Direct chemical method for sequencing RNA. Proc Natl Acad Sci USA 76: 1760–1764
Rast ATB (1977) Introductory remarks on strains of TMV infecting peppers in the Netherlands. In: Comptes rendues du 3me Congrés Eucarpia sur la Genetique et la Selection du Piment, Montlavet/Avignon, 1977. Eucarpia 77, Montlavet/Avignon, pp 83–84
Rietveld K, Linschooten K, Pleij CWA, Bosch L (1984) The three-dimensional folding of the tRNA-like structure of tobacco mosaic virus RNA. A new building principle applied twice. EMBO J 3: 2613–2619
Robinson DJ, Hamilton WDO, Harrison BD, Baulcombe DC (1987) Two anomalous tobravirus isolates: evidence for RNA recombination in nature. J Gen Virol 68: 2551–2561
Saito T, Yamanaka K, Watanabe Y, Takamatsu N, Meshi T, Okada Y (1989) Mutational analysis of the coat protein gene of tobacco mosaic virus in relation to hypersensitive response in tobacco plants with the N′ gene. Virology 173: 11–20
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenic trees. Mol Biol Evol 4: 406–425
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain termination inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Selassie KG, Dumas de Vaulx P, Marchoux G, Pochard E (1981) Le virus de la mosaïque du tabac chez le piment. I. Apparition en France du pathotype P1,2. Agronomie 1: 853–858
Solís I, García-Arenal F (1990) The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology 177: 553–558
Stoimenova E (1983) New strain of tobacco mosaic virus (TMV) in pepper. In: Proceedings of the 5th Eucarpia Meeting on Capsicum and Eggplant, Plovdiv, 1983. Eucarpia 83, Plovdiv/Bulgaria, pp 161–164
Takamatsu N, Watanabe Y, Meshi T, Okada Y (1990) Mutational analysis of the pseudoknot region in the 3′ noncoding region of tobacco mosaic virus RNA. J Virol 64: 3686–3693
Tóbiás I, Rast ATB, Maat DZ (1982) Tobamoviruses of pepper, eggplant and tobacco: comparative host reactions and serological relationships. Neth J Plant Pathol 88: 257–268
Van Belkum A, Abraham JP, Pleij CWA, Bosch L (1985) Five pseudoknots are present at the 204 nucleotides long 3′ noncoding region of tobacco mosaic virus RNA. Nucleic Acids Res 13: 7673–7686
Wetter C (1984) Serological identification of four tobamoviruses infecting pepper. Plant Dis 68: 597–599
Wetter C, Conti M (1988) Pepper mild mottle virus. AAB Descriptions of Plant Viruses, no 330
Wetter C, Conti M, Altschuh D, Tabillion R, Van Regenmortel MHV (1984) Pepper mild mottle virus, a tobamovirus infecting pepper cultivars in Sicily. Phytophatology 74: 405–410
Wetter C, Dore I, Bernard M (1987) Bell pepper mottle virus, a distinct tobamovirus infecting pepper. J Phytopathol 119: 333–344
Wilson V, Taylor P, Desselberger U (1988) Crossover regions in foot-and-mouth disease virus (FMDV) recombinants correspond to regions of high local secondary structure. Arch Virol 102: 131–139
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García-Luque, I., Ferrero, M.L., Rodríguez, J.M. et al. The nucleotide sequence of the coat protein genes and 3′ non-coding regions of two resistance-breaking tobamoviruses in pepper shows that they are different viruses. Archives of Virology 131, 75–88 (1993). https://doi.org/10.1007/BF01379081
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DOI: https://doi.org/10.1007/BF01379081