Summary
Morphometry, microfluorimetry and electron microscopy were used to investigate the size, ploidy level, and morphology of host nuclei in the ericoid mycorrhizal systemsCalluna vulgaris+Hymenoscyphus ericae andVaccinium myrtillus+H. ericae. Hypertrophy was observed in nuclei of mycorrhizal hair roots of both plants. This could not be explained by polyploidization but rather by a lower degree of chromatin condensation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barroso J, Pais MS (1990) Nuclear features in infected roots ofOphrys lutea Cav. (Orchidaceae). New Phytol 115: 93–98
Berta G, Bonfante-Fasolo P (1989) Anatomy and cytology of hair roots inCalluna vulgaris. Giorn Bot It 123: 9–24
—, Fusconi A, Sgorbati S, Trotta A, Scannerini S (1986) Preliminary results on the ploidy and fine structure of the nuclei of the host cells in a VA mycorrhiza. Giorn Bot Ital 120: 84–88
—, Gianinazzi-Pearson V, Gay G, Torri G (1988) Morphogenetic effects on endomycorrhiza formation on the root system ofCalluna vulgaris (L.) Hull. Symbiosis 5: 33–44
—, Sgorbati S, Trotta A, Fusconi A, Scannerini S (1989) Correlations between host-endophyte interactions and structural changes in host cell chromatin in a VA mycorrhiza. In: Nardon P, Gianinazzi-Pearson V, Grenier AM, Margulis L, Smith DC (eds) Endocytobiology IV. INRA Press, Paris, pp 145–148
— —, Soler V, Fusconi A, Trotta A, Citterio A, Bottone MG, Sparvoli E, Scannerini S (1990 a) Variations in chromatin structure in host nuclei of a vesicular arbuscular mycorrhiza. New Phytol 114: 199–205
- - Trotta A, Scannerini S (1990 b) Flow cytometry analysis of DNA and nuclear proteins in aPisum sativum L. VA mycorrhiza. In: Abstracts of the Eighth North American Conference on Mycorrhizae, Jackson, Wyoming, 5–8 September, 1990, 22
—, Fusconi A, Sgorbati S, Trotta A, Scannerini S (1991) Ultrastructural evidence for nucleus activation in infected cells of a VA mycorrhiza. Giorn Bot It 124: 658–660
Blair DA, Peterson RL, Bowley SR (1988) Nuclear DNA content in root cells ofLotus andTrifolium colonized by the VAM fungus,Glomus versiforme. New Phytol 109: 167–170
Bonfante-Fasolo P (1984) Anatomy and morphology. In: Powell CL, Bagyarai DJ (eds) VA mycorrhizas. CRC Press, Boca Raton, pp 5–33
—, Gianinazzi-Pearson V (1979) Ultrastructural aspects of endomycorrhiza in the Ericaceae. I. Naturally infected hair roots ofCalluna vulgaris L. Hull. New Phytol 83: 739–744
—, Berta G, Gianinazzi-Pearson V (1981) Ultrastructural aspects of endomycorrhiza in the Ericaceae. II. Host-endophyte relationships inVaccinium myrtillus L. New Phytol 89: 219–224
Deltour R, Mosen H (1987) Proposal for the macromolecular oranization of higher plant nucleolonema. Biol Cell 60: 75–86
Dumas E, Tahiri-Alaoni A, Gianinazzi S, Gianinazzi-Pearson V (1990) Observations on modifications in gene expression with VA endomycorrhiza development in tabacco: qualitative and quantitative changes in protein profiles. In: Narolon V, Gianinazzi-Pearson V, Grenier AM, Margulis L, Smith DC (eds) Endocytobiology IV. INRA Press, Paris, pp 153–157
Holley D, Peterson RL (1979) Development of a vesicular-arbuscular mycorrhiza in bean roots. Can J Bot 57: 1960–1978
Gianinazzi-Pearson V, Gianinazzi S (1988) Morphological integration and functional compatibility between symbionts in vesicular arbuscular mycorrhizal associations. In: Scannerini S, Smith D, Bonfante-Fasolo P, Gianinazzi-Pearson V (eds) Cell to cell signals in plant, animal and microbial symbiosis. Springer, Berlin Heidelberg New York Tokyo, pp 73–84 (NATO ASI Series, series H, vol 17)
Gifford EM, Nitsch JP (1969) Response of tobacco nuclei to growth substances. Planta 85: 1–10
Hadley G, Williamson B (1972) Features of mycorrhizal infection in some Malayan orchids. New Phytol 71: 1111–1118
Holley D, Peterson RL (1979) Development of a vesicular-arbuscular mycorrhiza in bean roots. Can J Bot 57: 1960–1978
Lafontaine JG, Lord A (1974) An ultrastructural and radioautographic study of the evolution of the interphase nucleus in plant meristematic cells (Allium porrum). J Cell Sci 14: 391–398
Mazzini G, Giordano P, Riccardi A, Montecucco CM (1983) A flow cytometric study of the propidium iodide staining kinetics of human leukocytes and its relationships with chromatin structure. Cytometry 3: 443–448
Nagl W (1978) Condensed interphase chromatin in plant and animal cell nuclei: fundamental differences. In: Nagl W, Hemleben V, Ehrendorfer F (eds) Chromatin: organization, evolution, function. Springer, Berlin Heidelberg New York, pp 247–260
Pearson V, Read DJ (1973) The biology of mycorrhiza in the Ericaceae. I. The isolation of the endophyte and synthesis of mycorrhizas in aseptic culture. New Phytol 72: 371–379
Protsenko MA, Shemakhanova MN (1971) Viranie mikorizoovrazvishevo griva no ratzeri iadria i iadrishka vkletan korniaPisum sativum. Mikol Fitopatol 5: 335–338
Read DJ (1974)Pezizella ericae sp. nov., the perfect state of a typical mycorrhizal endophyte of the Ericaceae. Trans Br Mycol Soc 63: 381–383
—, (1983) The biology of mycorrhiza in the Ericales. Can J Bot 61: 985–1004
Toth R, Miller RM (1984) Dynamics of arbuscule development and degeneration in aZea mays mycorrhiza. Amer J Bot 71: 449–460
Williams PH (1966) A cytochemical study of nuclear hypertrophy in clubroot of cabbage. Phytopathology 56: 521–524
Williamson B (1970) Induced DNA synthesis in orchid mycorrhiza. Planta 92: 347–354
—, Hadley G (1969) DNA content of nuclei in orchid protocorms symbiotically infected withRhizoctonia. Nature 222: 582–583
Woolhouse HW (1984) Sequences in plant cells. In: Davies I, Sigee DC (eds) Cell ageing and death. Cambridge University Press, Cambridge, pp 123–154
Wyss P, Mellor RB, Wiemken A (1990) Vesicular-arbuscular mycorrhizas of wild-type soybean and non-nodulating mutants withGlomus mosseae contain symbiosis-specific polypeptides (mycorrhizins), immunologically cross-reactive with nodulin. Planta 182: 22–26
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Berta, G., Sgorbati, S., Giaccone, P. et al. Nuclear morphology and ploidy level in infected and uninfected hair root cells ofCalluna vulgaris andVaccinium myrtillus mycorrhizae. Protoplasma 170, 160–165 (1992). https://doi.org/10.1007/BF01378790
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01378790