Summary
The distribution of Azotobacter was studied in the rhizosphere of some crop plants grown in this country. Any rhizosphere effect was very small and frequently Azotobacter was suppressed. No cells were recovered from root washings, indicating absence from the root surface. Azotobacter was absent from the rhizosphere in acid soils. Inoculation of seeds, roots, and soil resulted in establishment of high numbers of Azotobacter in the rhizospheres of plants grown in soil of pH above 6.5 and containing a natural Azotobacter population. Seed inoculation was the most practical and led to good establishment throughout the root system, including adventitious roots of wheat. The final population in the rhizosphere depended on the size and age of the initial inoculum applied to the seed. Best results came from spraying seeds with 14-day-old cultures and sowing the seed on the same day.
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References
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Brown, M.E., Burlingham, S.K. & Jackson, R.M. Studies onAzotobacter species in soil. Plant Soil 17, 320–332 (1962). https://doi.org/10.1007/BF01377671
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DOI: https://doi.org/10.1007/BF01377671