Skip to main content
SpringerLink
Log in

Diethylnitrosamine- and partial hepatectomy-induced decrease in α2u-globulin mRNA level in the rat liver

  • Rapid Communication
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

Changes in gene expression in the rat liver were investigated by analyzing cDNA libraries for liver mRNAs from adult male rats injected with a chemical carcinogen diethylnitrosamine (DEN). Differential screening using normal and DEN-treated liver cDNAs as probes demonstrated that some of the mRNA species had noticeably lower abundance in the DEN-treated liver than in the untreated liver. Surprisingly, most of those clones were found to code for α2u-globulin (A2uG), an abundant protein in the male rat liver. Further analysis by in situ hybridization revealed that the decrease in the A2uG mRNA level occurred in the area where liver cells were proliferating due to DEN treatment and/or partial hepatectomy (PH). The findings indicate coincidence of cell proliferation with a decrease in the A2uG gene expression in the adult male rat liver, implying that the A2uG-related change favors chemical carcinogen-induced cell growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

A2uG:

α2u-globulin

DEN:

diethylnitrosamine

PH:

partial hepatectomy

References

  • Colantuoni V, Romano V, Bensi G, Santoro C, Costanzo F, Raugei G, Cortese R (1983) Cloning and sequencing of a full length cDNA coding for human retinol-binding protein. Nucl Acids Res 11:7769–7776

    PubMed  Google Scholar 

  • Dietrich DR, Swenberg JA (1991) The presence of α2u-globulin is necessary ford-limonene promotion of male rat kidney tumors. Cancer Res 51:3512–3521

    PubMed  Google Scholar 

  • Farber E (1984) Cellular biochemistry of the stepwise development of cancer with chemicals. Cancer Res 44:5463–5474

    PubMed  Google Scholar 

  • Grisham JW (1962) A morphologic study of deoxyribonucleic acid synthesis and cell proliferation in regenerating rat liver; autoradiography with thymidine-H3. Cancer Res 22:842–849

    PubMed  Google Scholar 

  • Higgins GH, Anderson RM (1931) Experimental pathology of the liver I. Restoration of the liver of the white rat following partial surgical removal. Arch Pathol 12:186–202

    Google Scholar 

  • Kaumeyer JF, Polazzi JO, Kotick MP (1986) The mRNA for a proteinase inhibitor related to the HI-30 domain of inter-α-trypsin inhibitor also encodes α-1-microglobulin (protein HC). Nucl Acids Res 14:7839–7850

    PubMed  Google Scholar 

  • Kohnoe S, Maehara Y, Endo H (1988) Difference in gene expression between azo-dye-induced primary hepatomas and corresponding transplantable ascites hepatoma lines in rats. Oncology 45:51–56

    PubMed  Google Scholar 

  • MacInnes JI, Nozik ES, Kurtz DT (1986) Tissue-specific expression of the rat alpha2u globulin gene family. Mol Cell Biol 6:3563–3567

    PubMed  Google Scholar 

  • Nakhasi HL, Lynch KR, Dolan KP, Unterman R, Antakly T, Teigelson P (1982) Modifications in α2u globulin gene structure, transcription, and mRNA transcription in hepatomas. J Biol Chem 257:2726–2729

    PubMed  Google Scholar 

  • Pervaiz S, Brew K (1987) Homology and structure-function correlations between α1-acid glycoprotein and serum retinol-binding protein and its relatives. FASEB J 1:209–214

    PubMed  Google Scholar 

  • Pevsner J, Reed RR, Feinstein PG, Snyder SH (1988) Molecular cloning or odorant-binding protein: member of a ligand carrier family. Science 241:336–339

    PubMed  Google Scholar 

  • Pitot HC, Beer D, Hendrich S (1988) Multistage carcinogenesis: the phenomenon underlying the theories. In: Iversen OH (ed) Theories of Carcinogenesis. Hemisphere, Washington, pp 159–177

    Google Scholar 

  • Rappaport AM, Borowy AJ, Lougheed WM, Lotto WN (1954) Subdivision of hexagonal liver lobules into a structural and functional unit. Anat Rec 119:11–33

    PubMed  Google Scholar 

  • Roy AK, Chatterjee B, Demyan WF, Milin BS, Motwani NM, Nath TS, Schiop MJ (1983) Hormone and age-dependent regulation of α2u-globulin gene expression. Recent Prog Hormone Res 39:425–457

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning. A Laboratory Manual/Second Edition. Cold Spring Harbor Laboratory Press, NY

    Google Scholar 

  • Schmid K, Kaufman H, Isemura S, Nanno S (1973) Structure of α1-acid glycoprotein. The complete amino acid sequence, multiple amino acid substitutions, and homology with the immunoglobulins. Biochemistry 12:2711–2724

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontology, Sakae-cho 35-2, Itabashi-ku, 173, Tokyo, Japan

    Koozi Matuoka

  2. Carcinogenesis Research Unit, School of Pathology, University of New South Wales, 2033, Kensington, NSW, Australia

    Koozi Matuoka, Irit Markus, Angelina Wong & Garry J. Smith

Authors
  1. Koozi Matuoka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Irit Markus
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Angelina Wong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Garry J. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matuoka, K., Markus, I., Wong, A. et al. Diethylnitrosamine- and partial hepatectomy-induced decrease in α2u-globulin mRNA level in the rat liver. J Cancer Res Clin Oncol 119, 572–575 (1993). https://doi.org/10.1007/BF01372719

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01372719

Key words

Search

Navigation