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Growth and polyamine metabolism inPyrenophora avenae exposed to cyclohexylamine and norspermidine

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Summary

The effectiveness of inhibitors of polyamine biosynthesis in controlling plant pathogenic fungi is well established. The spermidine synthase inhibitor cyclohexylamine (CHA) and the spermidine analogue norspermidine were evaluated againstin vitro growth of the oat stripe pathogenPyrenophora avenae. Mycelial growth was reduced by 55% upon exposure to 2.0mM CHA while the same concentration of norspermidine reduced growth by 63%. Neither inhibitor had any effect on ODC or AdoMetDC activities, nor the flux of label from ornithine through to the polyamines. Levels of free polyamines in fungal tissue exposed to 0.01 mM norspermidine were unaltered, although 1.0mM CHA did produce a 75% increase in fungal putrescine content. These data suggest that CHA and norspermidine do not reduce fungal growth as a result of a perturbation in polyamine biosynthesis.

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Abbreviations

ODC:

ornithine decarboxylase

ADC:

arginine decarboxylase

AdoMetDC:

S-adenosylmethionine decarboxylase

DFMO:

adifluoromethylornithine

CHA:

cyclohexylamine

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Authors and Affiliations

  1. Plant Science Department, The Scottish Agricultural College, Auchincruive, Ayr, KA6 5HW, UK

    C. A. Mackintosh & D. R. Walters

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  1. C. A. Mackintosh
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  2. D. R. Walters
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Mackintosh, C.A., Walters, D.R. Growth and polyamine metabolism inPyrenophora avenae exposed to cyclohexylamine and norspermidine. Amino Acids 13, 347–354 (1997). https://doi.org/10.1007/BF01372598

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  • DOI: https://doi.org/10.1007/BF01372598

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