Summary
A sensitive immunodetection method for Alpha-2-HS glycoprotein (AHSG) after ultrathin layer polyacrylamide gel isoelectric focusing has been applied to a family study of 126 matings including 292 offspring. Formal genetic studies are in agreement with an autosomal mode of inheritance for this system. A population study of 506 unrelated individuals from Galicia (NW Spain) gave the following frequencies: AHSG*1= 0.7559 and AHSG*2 = 0.2441, which correspond to a exclusion chance for non-fathers of 0.1505.
Zusammenfassung
Eine empfindliche immunologische Nachweismethode für die Alpha-2-HS-Glykoprotein (AHSG)-Diagnose nach Ultradünnschicht-Polyacryla-mid-Gel-Isoelektrofokussierung wurde in einer Familienstudie angewandt, welche 126 Elternverbindungen und 292 Kinder umfaßte. Die formalgenetischen Untersuchungen stehen mit der Annahme eines autosomalen Erbgangs in Übereinstimmung. Eine populationsgenetische Untersuchung von 506 unverwandten Personen von Galicia (Nordwest-Spanien) ergab die folgenden Genfrequenzen: AHSG*1= 0,7559 and AHSG*2 = 0,2441; diese Frequenzen entsprechen einer Ausschließungschance für Nicht-Väter von 0,1505.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abe S, Hiraiwa K, Sebetan IM (1987) Distribution of α2HS-Glycoprotein types in Egyptian. J Anthrop Soc Nippon 95:137–139
Anderson NL, Anderson NG (1977) High resolution two-dimensional electrophoresis in human plasma proteins. Proc Natl Acad Sci USA 74:5421–5425
Boutin B, Feng SH, Arnaud P (1985) The genetic polymorphism of alpha-2-HS glycoprotein: study by ultrathin-layer isoelectric focusing and immunoblot. Am J Hum Genet 37:1098–1105
Budowle B, Scott E (1985) Transferrin subtyping of human blood stains. Forensic Sci Int 28:269–275
Cox DW, Andrews BJ (1983) Silver stain immunofixation for α2HS-glycoprotein: a new method for detection of protein heterogeneity. In: Stathokos D (ed) Electrophoresis '82. Walter de Gruyter, Berlin, pp 243–247
Cox DW, Andrews BJ, Wills DE (1986) Genetic polymorphism of α2HS glycoprotein. Am J Hum Genet 38:699–706
Fukuma Y, Kashimura S, Nakano B, Umetsu K, Yuasa I, Nakasono I (1991) ‘Genetic polymorphism of Alpha-2-HS-glycoprotein: four new alleles and allele frequencies in Japanese. Hum Hered 41:89–92
Luckenbach C, Kömpf J, Ritter H (1988) Polymorphism of AHSglycoprotein: comparison of different IEF and detection techniques. In: Mayr WR (ed) Advances in Forensic Haemogenetics 2. Springer, Berlin Heidelberg New York, pp 235–239
Olaisen B, Mevag B, Teisberg P, Gedde-Dahl TJ, Helland P, Siverts A, Jonassen R, Hjalmarsson K, Swan T (1981) Plasma protein polymorphisms in two-dimensional electrofocusing/ SDS electrophoresis. 9. Internationale Tagung der Gesellschaft für forensische Blutgruppenkunde e. V. Referate. Bern 29/9 - 3/10, pp 543-555
Umetsu K, Kashimura S, Ikeda N, Suzuki T (1983) Classification of α2-HS Glycoprotein (α2HS) types by isoelectric focusing. Z Rechtsmed 91:33–35
Weidinger S, Schwarzfisher F, Burgemeister R, Cleve H (1984) Genetic Alpha-2-HS glycoprotein phenotypes demonstrated by isoelectric focusing and immunofixation. In: Neuhoff V (ed) Electrophoresis'84. Verlag Chemie, Weinheim, pp 487–490
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cameselle, F.D., Teixeira, C., Parra, E. et al. Formal and population genetic studies of AHSG: Further data from Galicia. Int J Leg Med 105, 51–52 (1992). https://doi.org/10.1007/BF01371239
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01371239