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Some technical parameters influencing the precision and accuracy of fragment size determination for RFLP's

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Summary

A comparison was carried out between 3 computer-assisted systems for the estimation of DNA fragment length: (1) the “Digitab” system (developed at our institute), (2) the FBI analysis system (FBI Quantico, USA) and (3) the BioImage system (Waters/Millipore, USA). Both the FBI system and the Biolmage system were found to be much more precise than the manual Digitab system. In an additional experiment, a possible influence of the field strength on the “accuracy” of fragment size measurement was checked. Lowering the field strength to approx. 1 V/cm led to a statistically significant increase in the measured fragment size.

Zusammenfassung

Drei computer-unterstützte Systeme zur DNA-Fragmentlängenbestimmung wurden verglichen: (1) Das „Digitab-System” (entwickelt im Institut für Rechtsmedizin, Münster, FRG), (2) das „FBI-Analysis-System” (FBI, Quantico, USA) und (3) das Biolmage-System (Waters/Millipore, USA). Das FBI-Analysis-System und das BioImage-System arbeiteten präziser als das halbautomatische Digitab-System. In einem zusätzlichen Experiment wurde der mögliche Einfluß der Feldstärke auf die „Richtigkeit” der Fragmentlangenbestimmung untersucht. Eine Reduzierung der Feldstärke auf etwa 1 V/cm führte zu signifikant höheren Werten bei der Fragmentlängenbestimmung.

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Authors and Affiliations

  1. Institut für Rechtsmedizin, Universität Münster, Von-Esmarch-Strasse 86, W-4400, Münster, Bundesrepublik Deutschland

    C. Puers, P. Wiegand & B. Brinkmann

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  1. C. Puers
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  2. P. Wiegand
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  3. B. Brinkmann
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Puers, C., Wiegand, P. & Brinkmann, B. Some technical parameters influencing the precision and accuracy of fragment size determination for RFLP's. Int J Leg Med 105, 31–34 (1992). https://doi.org/10.1007/BF01371234

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  • DOI: https://doi.org/10.1007/BF01371234

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