Skip to main content
SpringerLink
Log in

Evaluation of three serological tests for detection of anti-candidal antibodies in diagnosis of invasive candidiasis

Mycopathologia volume 126, pages 3–7 (1994)Cite this article

Abstract

Immunoblot detection of antibody against 47 KD cytoplasmic antigen ofCandida albicans was evaluated in diagnosis of invasive candidiasis and compared to whole cell agglutination and gel diffusion tests for detection of anticandidal antibody in 64 patients. The patients included 17 with culture proved candidemia, 34 with significant candiduria (more than 10,000 colony forming units per ml of urine) and 13 with nonsignificant candiduria. Antibody against 47 KD antigen was found to be the best indicator for diagnosis of invasive candidiasis even in patients with malignancy. The sensitivity of this procedure was 82.4%, specificity 86.7%, positive predictive value 77.8%, negative predictive value 89.7% and efficacy 85.1%. The gel diffusion procedure lacked in sensitivity whereas whole cell agglutination lacked in specificity. Detection of antibody against 47 KD antigen proved to be a valuable adjunct in the diagnosis of invasive candidiasis.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

References

  1. Walsh TJ, Hathorn JW, Sebel JD, et al. Detection of circulating candida enolase by immunoassay in patients with cancer and invasive candidiasis. N Engl J Med 1991; 324: 1026–31.

    PubMed  Google Scholar 

  2. Odds FC (ed). Candida and candiosis. London: Baillière Tindall, 1988; 1–278.

    Google Scholar 

  3. de Repentigny L, Reiss E. Current trends in immunodiagnosis of candidiasis and aspergillosis. Rev Infect Dis 1984; 6: 301–312.

    PubMed  Google Scholar 

  4. Gentry LO, Price MF. Urinary and genital candida infection. In: Bodey GP, Fainstein V (ed), Candidiasis. New York, Raven Press, 1985; 174.

    Google Scholar 

  5. Bodey GP, Finstein V (ed), Candidiasis. New York: Raven Press, 1985; 146.

    Google Scholar 

  6. Bodey GP. Fungal infections complicating acute leukaemia. J Chron Dis 1966; 19: 667–87.

    PubMed  Google Scholar 

  7. Taschdjian CL, Seelig MS, Kozinn PJ. Serological diagnosis of candidal infections. CRC Cr Rev Clin Lab Sci 1973; 54: 19–59.

    Google Scholar 

  8. Platenkamp GJ, Vanduin AM, Porsius JC, Schouten HJA, Zondervan PE, Michel MF. Diagnosis of invasive candidiasis in patients with and without signs of immune deficiency: A comparison of six detection methods in human serum. J Clin Pathol 1987; 40: 1162–67.

    PubMed  Google Scholar 

  9. Mathews RC, Burnie JP. Diagnosis of systemic candidiasis immunodominant 47-Kilodalton antigen. J Clin Microbiol 1988; 26: 459–63.

    PubMed  Google Scholar 

  10. Ruchel R, Boning-stutzer B, Mari A. A synoptical approach to the diagnosis of candidosis, relying on serological antigen and antibody tests, on culture and evaluation of clinical data. Mycoses 1988; 31: 87–106.

    PubMed  Google Scholar 

  11. Ruchel R. Candidosis: Diagnostic tools in the laboratory. Mycoses 1989; 32: 18–22.

    PubMed  Google Scholar 

  12. Quindos G, Ponton J, Cisterna R, Mackenzie DWR. Value of detection of antibodies ofCandida albicans germ tube in the diagnosis of systemic candidosis. Eur J Clin Microbiol Infect Dis 1990; 9: 178–83.

    PubMed  Google Scholar 

  13. Strockbine NA, Largen MT, Zweibel SM, Buckley HR. Identification and molecular weight characterization of antigens fromCandida albicans that are recognised by human sera. Infect Immun 1984; 43: 715–21.

    PubMed  Google Scholar 

  14. Buckley HR. Identification of yeasts In: Evans EGV, Richardson MD (ed). Medical Mycology: A practical approach. Oxford: IRL Press, 1989: 97–109.

    Google Scholar 

  15. Palmer DF, Kaufman L, Kaplan WM, Cavallaro J. Serodiagnosis of mycotic diseases. Springfield, IL: Charles C. Thomas, 1977: 44.

    Google Scholar 

  16. Merz WG, Evans GL, Shadomy S, et al. Laboratory evaluation of serological tests for systemic candidiasis: A cooperative study. J Clin Microbiol 1977; 5: 596–603.

    PubMed  Google Scholar 

  17. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nintracellulose sheets: Procedure and applications. Proc Natl Acad Sci 1979; 76: 4350–54.

    PubMed  Google Scholar 

  18. Lee KL, Buckley HR, Campbell CC. An amino acid liquid synthetic medium for the development of mycelial and yeast forms ofCandida albicans. Sabouraudia 1975; 13: 148–53.

    PubMed  Google Scholar 

  19. Glew RH, Buckley HR, Rosen HM, Moellering RC, Fischer JE. Serologic tests in diagnosis of systemic candidiasis. Am J Med 1978; 64: 586–91.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Microbiology, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, India

    A. Chakrabarti, P. Roy, D. Kumar & D. Panigrahi

  2. Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, India

    B. K. Sharma

  3. Department of Nephrology, Postgraduate Institute of Medical Education and Research, 160012, Chandigarh, India

    K. S. Chugh

Authors
  1. A. Chakrabarti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Roy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. K. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. S. Chugh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. D. Panigrahi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Chakrabarti, A., Roy, P., Kumar, D. et al. Evaluation of three serological tests for detection of anti-candidal antibodies in diagnosis of invasive candidiasis. Mycopathologia 126, 3–7 (1994). https://doi.org/10.1007/BF01371166

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01371166

Key words

search

Navigation