International Journal of Legal Medicine

, Volume 108, Issue 2, pp 68–74 | Cite as

Validation of mitochondrial DNA sequencing for forensic casework analysis

  • Mark R. Wilson
  • Joseph A. DiZinno
  • Deborah Polanskey
  • Jeri Replogle
  • Bruce Budowle
Original Article


Two sets of studies were performed to evaluate the forensic utility of sequencing human mitochondrial DNA (mtDNA) derived from various tissues and amplified by the polymerase chain reaction (PCR). Sequencing was performed on a Perkin-Elmer/Applied Biosystems Division (PE/ABD) automated DNA sequencer (model 373A). The first set of experiments included typical validation studies that had previously been conducted on forensic DNA markers, such as: chemical contaminant effects on DNA from blood and semen and the effect of typing DNA extracted from body fluid samples deposited on various substrates. A second set of experiments was performed strictly on human hair shafts. These studies included typing mtDNA from hairs that were: (1) from different body areas, (2) chemically treated, (3) from deceased individuals, and (4) deliberately contaminated with various body fluids. The data confirm that PCR-based mtDNA typing by direct automated sequencing is a valid and reliable means of forensic identification.

Key words

Mitochondrial DNA Forensics Hair Human polymorphisms Sequencing 


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Mark R. Wilson
    • 2
  • Joseph A. DiZinno
    • 2
  • Deborah Polanskey
    • 2
  • Jeri Replogle
    • 1
  • Bruce Budowle
    • 1
  1. 1.Forensic Science Research and Training CenterFBI AcademyQuanticoUSA
  2. 2.Hairs and Fibers Unit, FBI LaboratoryFBI HeadquartersWashingtonUSA

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