Skip to main content
Log in

Analysis of a short tandem repeat locus on chromosome 19 (D19S253)

  • Original Article
  • Published:
International Journal of Legal Medicine Aims and scope Submit manuscript

Abstract

A tetranucleotide tandem repeat locus on chromosome 19 (D19S253) was analysed. PCR products were detected by denaturing polyacrylamide gels with fluorescent-based technology. This study has confirmed a polymorphism with 9 alleles ranging from 209 to 241 by with a simple repeat structure arranged from 7 to 15 repeats. Family studies confirmed mendelian inheritance of alleles. The efficiency on DNA extracted from bloodstains and cigarette butts has been evaluated. The protocol has shown sensitivity and reproducibility.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Urquhart A, Kimpton CP, Downes TJ, Gill P (1994) Variation in short tandem repeat sequences — a survey of twelve microsatellite loci for use as forensic identification markers. Int J Legal Med 107:13–20

    PubMed  Google Scholar 

  2. Moeller A, Meyer E, Brinkmann B (1994) Different types of structural variation in STRs: HumFES/FPS, HumVWA and D21S11 Int J Legal Med 106:319–323

    PubMed  Google Scholar 

  3. Weber JL, Wang Z, Hansen K, Stephenson M, Kappel C, Salzman S, Wilkie PJ, Keats B, Dracopoli NC, Brandriff BF, Olsen AS (1993) Evidence for human meiotic recombination interference obtained through construction of a short tandem repeatpolymorphism linkage map of chromosome 19. Am J Hum Genet 53:1079–1095

    PubMed  Google Scholar 

  4. Urquhart A, Chin CT, Clayton T, Downes T, Frazier R, Jones S, Kimpton C, Laren MV, Millican E, Oldroyd N, Thompson C, Watson S, Whitaker J, Gill P (1995) Multiplex STR systems with fluorescent detection as human identification markers. Proceedings of the Fifth International Symposium on Human Identification 1994. Promega Corporation pp 73–83

  5. DNA Commission of the International Society for Forensic Haemogenetics (1994) DNA recommendations — 1994 report concerning further recommendations of the DNA Commission of the ISFH regarding PCR-based polymorphisms in STR (short tandem repeat) systems. Forensic Sci Int 69: 103–104

    Google Scholar 

  6. Lygo JE, Johnson PE, Holdaway DJ, Woodroffe S, Whitaker JP, Clayton TM, Kimpton CP, Gill P (1994) The validation of short tandem repeat (STR) loci for use in forensic casework. Int J Legal Med 107:77–89

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

De Stefano, F., Casarino, L., Costa, M.G. et al. Analysis of a short tandem repeat locus on chromosome 19 (D19S253). Int J Leg Med 108, 256–258 (1996). https://doi.org/10.1007/BF01369821

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01369821

Key words

Navigation