Abstract
Phencyclidine (PCP) can be detected in body fluids with very high sensitivity by gas chromatography (GC) with surface ionization detection (SID) using pethidine as internal standard. PCP was extracted with Sep-Pak C18 cartridges from whole blood and urine samples, which gave clean extracts. The calibration curve for spiked whole blood was linear in the range 1.25–20 ng/ml. The detection limit of PCP was approximately 15 pg on-column (0.75 ng/ml sample), which was much lower than by GC-nitrogen phosphorus detection. The recovery of PCP and pethidine from spiked whole blood or urine samples was above 85%. This method seems very useful for the determination of PCP in forensic and clinical toxicology.
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References
Gilman AG, Rall TW, Nies AS, Taylor P (eds) (1990) Goodman and Gilman's the pharmacological basis of therapeutics, 12th edn. Pergamon, New York, pp 557–558
Zukin SR, Zukin RS (1992) Phencyclidine. In: Lowinson JH, Ruiz P, Millman RB (eds) Substance abuse: a comprehensive textbook. Williams & Wilkins, Baltimore, pp 290–302
Fujii T, Arimoto H (1985) New sensitive and selective detector for gas chromatography: surface ionization detector with a hot platinum emitter. Anal Chem 57: 2625–2628
Fujii T, Arimoto H (1992) The surface ionization detector. In: Hill H, McMinn DG (eds) Detectors for capillary chromatography. Chemical analysis series, Vol. 121. Wiley, Chichester, pp 169–191
Froelich PM, Ross G (1977) Separation and detection of phencyclidine in urine by gas chromatography. J Chromatogr 137: 135–143
Caplan YH, Orloff KG, Thompson BC, Fisher RS (1979) Detection of phencyclidine in medical examiner's cases. J Anal Toxicol 3: 47–52
Clardy DO, Cravey RH, MacDonald BJ, Wiersema SJ, Pearce DS, Reagle JL (1979) The phencyclidine-intoxicated driver. J Anal Toxicol 3: 238–241
Lewellen LJ, Solomons ET, O'Brien FL (1979) Nitrogen-sensitive gas chromatographic detection and quantitation of nanogram levels of phencyclidine in whole blood. J Anal Toxicol 3: 72–75
Bailey DN, Guba JJ (1980) Gas-chromatographic analysis for phencyclidine in plasma, with use of a nitrogen detector. Clin Chem 26:437–440
Miceli JN, Bowman DB, Aravind MK (1981) An improved method for the quantitation of phencyclidine (PCP) in biological samples utilizing nitrogen-detection gas chromatography. J Anal Toxicol 5: 29–32
Werner M, Hertzman M, Pauley CJ (1986) Gas-liquid chromatography of phencyclidine in serum, with nitrogen-phosphorus detection. Clin Chem 32: 1921–1924
Verebey K, DePace A (1988) Rapid confirmation of enzymemultiplied immunoassay test (EMIT) for phencyclidine-positive urine samples with capillary gas chromatography-nitrogenphosphorus detection. J Chromatogr 427: 151–156
Lin DCK, Fentiman Jr AF, Foltz RL (1975) Quantitation of phencyclidine in body fluids by gas chromatography chemical ionization mass spectrometry and identification of two metabolites. Biomed Mass Spectrom 2: 206–241
Kammerer RC, Stefano ED, Schmitz D (1980) A gas liquid chromatography assay for phencyclidine and its metabolites. J Anal Toxicol 4: 293–298
Cone EJ, Buchwald W, Yousefnejad D (1981) Simultaneous determination of phencyclidine and monohydroxylated metabolites in urine of man by gas chromatography-mass fragmentography with methane chemical ionization. J Chromatogr 223: 331–339
El Sohly MA, Little Jr TL, Mitchell JM, Paul BD, Mell Jr LD, Irving J (1988) GC/MS analysis of phencyclidine acid metabolite in human urine. J Anal Toxicol 12: 180–182
Kandiko CT, Browning S, Cooper T, Cox WA (1990) Detection of low nanogram quantities of phencyclidine extracted from human urine. J Chromatogr 528: 208–213
Wu AHB, Onigbinde TA, Wong SS, Johnson KG (1992) Evaluation of full-scanning GC/ion trap MS analysis of NIDA drugs-of-abuse testing in urine. J Anal Toxicol 16: 202–206
Stevenson CC, Cibull DL, Platoff GE, Bush DM, Gere JA (1992) Solid phase extraction of phencyclidine from urine followed by capillary gas chromatography/mass spectrometry. J Anal Toxicol 16: 337–339
Cook CE, Brine DR, Jeffcoat AR, Hill JM, Wall ME, Perez Reyes M, Di Guiseppi SR (1982) Phencyclidine disposition after intravenous and oral doses. Clin Pharmacol Ther 31: 625–634
Owens SM, Woodworth J, Mayersohn M (1982) Radioimmunoassay for phencyclidine (PCP) in serum. Clin Chem 28: 1509–1513
Moriya F, Chan KM, Noguchi TT, Wu PYK (1994) Testing for drugs of abuse in meconium of newborn infants. J Anal Toxicol 18: 41–45
Hattori H, Yamada T, Suzuki O (1994) Gas chromatography with surface ionization detection in forensic analysis. J Chromatogr A 674: 15–23
Kumazawa T, Seno H, Suzuki O (1990) Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of bromosovalum. J Anal Toxicol 16: 163–165
Hattori H, Yamamoto S, Iwata M, Takeshima E, Yamada T, Suzuki O (1992) Sensitive determination of phenothiazines in body fluids by gas chromatography with surface ionization detection. J Chromatogr 579: 247–252
Javitt DC, Zukin SR (1991) Recent advances in the phencyclidine model in schizophrenia. Am J Psychiat 148: 1301–1308
Moffat AC, Jackson JV, Moss MS, Windrop B (eds) (1986) Clarke's isolation and identification of drugs, 2nd edn. Pharmaceutical Press, London, pp 874–875
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Ishii, A., Seno, H., Nishikawa, M. et al. Simple and sensitive detection of phencyclidine in body fluids by gas chromatography with surface ionization detection. Int J Leg Med 108, 244–247 (1996). https://doi.org/10.1007/BF01369818
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DOI: https://doi.org/10.1007/BF01369818