Summary
The muscle proteins actin, myosin, desmin and myoglobin were investigated in traumatically damaged human and animal skeletal muscle using an immunohistochemical PAP-method. A depletion of all the proteins investigated was observed in muscle fibres damaged in the antemortem period. The antigens could however also be demonstrated in the otherwise empty sarcolemma, the discoid disintegration zones of the fibres and between the fibres. The depletion begins immediately after the trauma and myoglobin is the first to be affected. No such changes could be observed after post mortem muscle damage. The antigens could be demonstrated until 72 hours postmortem. The demonstration of protein depletion is an important addition to the light microscopical findings in vital muscle alterations.
Zusammenfassung
An traumatisch geschädigter menschlicher und bei Tierexperimenten gewonnener Skelettmuskulatur wurden immunhistochemisch mit der PAP-Methode die Muskelproteine Aktin, Myosin, Desmin und Myoglobin dargestellt. Bei vital traumatisierter Muskulatur kommt es zu einer Depletion aller untersuchter Proteine aus der verletzten Muskelfaser. Die Antigene können aber in den sonst leeren Sarkolemm-Schläuchen, den diskoiden Faserzerfallszonen und auch außerhalb der Fasern nachgewiesen werden. Die Depletion beginnt Minuten nach dem Trauma, am frühesten beim Myoglobin. Bei postmortaler Traumatisierung waren die Veränderungen nicht nachweisbar. Die Antigene waren bis 72 Stunden postmortal stabil. Der Nachweis der Muskelprotein-Depletion stellt eine wichtige Ergänzung zu den lichtoptischen Kriterien vitaler Muskelfaseralterationen dar.
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Fechner, G., Hauser, R., Sepulchre, M.A. et al. Immunohistochemical investigations to demonstrate vital direct traumatic damage of skeletal muscle. Int J Leg Med 104, 215–219 (1991). https://doi.org/10.1007/BF01369810
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DOI: https://doi.org/10.1007/BF01369810