Abstract
The effects of the pyridazinone compound SAN 9785 on the photosynthetic competence of leaves, on the photochemical activity of isolated thylakoids and on the formation and spectral properties of chlorophyll-protein complexes were studied during a 72-h greening period of detached etiolated leaves of barley (Hordeum vulgare L. cv. Horpácsi kétsoros). It was established that i) the photosynthetic capacity of the leaves decreased considerably (by 80 and 90%, as determined by14CO2 fixation and fast fluorescence induction measurements, respectively); ii) the photochemical activity of isolated thylakoids from water to potassium ferricyanide and from dichlorophenol indophenol/ascorbate to methylviologen exhibited only slight reductions when expressed on a chlorophyll basis compared with the control; iii) the slow fluorescence induction curves of the treated leaves demonstrated the presence of a peculiar fluorescence component interrupting the quenching of fluorescence at around 1 min illumination; iv) a shortage of the chlorophyll-protein complex of photosystem I (CPI) occurred with a higher content of the monomer of the light harvesting complex in the thylakoids of treated leaves; and v) the fluorescence spectrum of the CPI band present in treated leaves indicates the destruction of the structural integrity of this complex during isolation from the membrane.
Similar content being viewed by others
Abbreviations
- Chl:
-
chlorophyll
- CPI, CPII:
-
chlorophyll-protein complexes of the reaction centres of PSI and PSII
- DCMU:
-
3-(3′,4′-dichlorophenyl)-1,1-dimethylurea
- DPIP:
-
2,6-dichlorophenol indophenol
- DPIPH2 :
-
chemically reduced form of DPIP
- F o :
-
fluorescence of constant yield
- F v :
-
fluorescence of variable yield
- F i ,F m :
-
mitial and maximum yield of fluorescence
- LHCP3 :
-
monomer of the light-harvesting complex
- LHCP2 and LHCP1 :
-
oligomers of the light-harvesting complex LHCP3
- PSI, PSII:
-
photosystems I, II
- SAN 9785:
-
4-chloro-5-(dimethylamino)-2-phenyl-3(2H)-pyridazinone, also known as BASF 13-338
- SDS-PAGE:
-
sodium dodecyl sulphate-polyacrylamide gel electrophoresis
References
Anderson, J.M. (1980) P700 content and polypeptide profile of chlorophyll-protein complexes of spinach and barley thylakoids. Biochim. Biophys. Acta591, 113–126
Anderson, J.M. (1982) The role of chlorophyll-protein complexes in the function and structure of chloroplast thylakoids. Mol. Cell. Biochem.46, 161–172
Anderson, J.M., Waldron, J.C., Thorne, S.W. (1978) Chlorophyll-protein complexes of spinach and barley thylakoids. Spectral characterization of six complexes resolved by an improved electrophoretic procedure. FEBS Lett.92, 227–233
Argyroudi-Akoyunoglou, J. (1984) The 77K fluorescence spectrum of the photosystem I pigment-protein complex CPIa. FEBS Lett.171, 47–53
Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenol oxidase inBeta vulgaris. Plant Physiol.24, 1–15
Avron, M., Krogmann, D.W., Jagendorf, A.T. (1958) The relation of the photosynthetic phosphorylation to the Hill reaction. Biochim. Biophys. Acta30, 144–153
Bose, S., Mannan, R.M., Arntzen, C.J. (1984) Increased synthesis of photosystem II inTriticum vulgare when grown in the presence of BAS 13-338. Z. Naturforsch.39c, 510–513
Dominy, P., Williams, W.P. (1985) The relationship between changes in the redox state of plastoquinone and control of excitation energy distribution in photosynthesis. FEBS Lett.179, 321–324
Goldbeck, J.H., Kok, B. (1979) Redox titration of electron acceptor Q and the plastoquinone pool in photosystem II. Biochim. Biophys. Acta547, 347–360
Good, N.E. (1960) Re-assessment of the stoichiometry of photophosphorylation. Nature188, 661–663
Govindjee, Papageorgiou, G. (1971) Chlorophyll fluorescence and photosynthesis: Fluorescence transients. In: Photophysiology, vol. 6, pp. 1–46, Giese, A., ed. Academic Press, New York London
Hilton, J.L., Scharen, A.L., St. John, J.B., Moreland, D.E., Norris, K.H. (1969) Modes of action of pyridazinone herbicides. Weed Sci.17, 541–547
Laskay, G., Farkas, T., Lehoczki, E., Gulya, K. (1983) Effects of pyridazinone herbicides during chloroplast development in detached barley leaves. II. Effects on lipid content, fatty acid composition and ultrastructure of chloroplasts. Z. Naturforsch.38c, 741–747
Laskay, G., Lehoczki, E. (1986) Correlation between linolenic acid deficiency in chloroplast membrane lipids and decreasing photosynthetic activity in barley. Biochim. Biophys. Acta849, 77–84
Leech, R.M., Walton, C.A., Baker, N.R. (1985) Some effects of 4-chloro-5-(dimethylamino)-2-phenyl-3(2H)-pyridazin-one (SAN 9785) on the development of chloroplast thylakoid membranes inHordeum vulgare L. Planta165, 277–283
Lem, N.W., Williams, J.P. (1981) Desaturation of fatty acids associated with monogalactosyl diacylglycerol: the effects of SAN 6706 and SAN 9785. Plant Physiol.68, 944–949
Lichtenthaler, H.K., Park, R.B. (1963) Chemical composition of chloroplast lamellae from spinach. Nature198, 1070–1072
Mannan, R.M., Bose, S. (1985) BASF 13.338 induced changes in structure and function of the photosynthetic apparatus of wheat seedlings. Photochem. Photobiol.41, 63–72
Papageorgiou, G. (1975) Chlorophyll fluorescence: An intrinsic probe of photosynthesis. In: Bioenergetics of photosynthesis, pp. 319–371, Govindjee, ed. Academic Press, New York London
Plesničar, M., Bendall, D.S. (1973) The photochemical activities and electron carriers of developing barley leaves. Biochem. J.136, 803–812
Quinn, P.J., Williams, W.P. (1978) Plant lipids and their role in membrane function. Prog. Biophys. Mol. Biol.34, 109–173
Rakhimberdieva, M.G., Lehoczki, E., Karapetyan, N.V., Krasnovsky, A.A. (1982) Effect of pyridazinone herbicides and cerulenin on the biosynthesis and function of photosystem II in barley leaves. [In Russ.] Biokhimija47, 637–645
Santarius, K.A. (1984) The role of the chloroplast coupling factor in the inactivation of thylakoid membrane at low temperatures. Physiol. Plant.61, 591–598
Sárvári, É., Nyitrai, P., Gyüre, K. (1984) Chlorophyll-protein derivative of the peripheral light-harvesting antenna of photosystem I. Photobiochem. Photobiophys.8, 229–237
St. John, J.B. (1976) Manipulation of galactolipid fatty acid composition with substituted pyridazinones. Plant Physiol.57, 257–259
Szigeti, Z., Tóth, É., Paless G. (1982) Mode of action of photosynthesis inhibiting 4-hydroxy-benzonitriles containing nitro group. Photosynth. Res.3, 347–356
Van Rensen, J.J.S., Van der Vet, W., Van Vliet, W.P.A. (1977) Inhibition and uncoupling of electron transport in isolated chloroplasts by the herbicide 4,6-dinitro-o-cresol. Photochem. Photobiol.25, 579–583
Wild, A., Krebs, B., Rühle, W. (1980) Methodical studies in the separation of chlorophyll-protein complexes by polyacrylamide gel electrophoresis. Z. Pflanzenphysiol.100, 1–13
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Laskay, G., Lehoczki, E., Dobi, A.L. et al. Photosynthetic characteristics of detached barley leaves during greening in the presence of SAN 9785. Planta 169, 123–129 (1986). https://doi.org/10.1007/BF01369783
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01369783