Abstract
The effects of the pyridazinone compound SAN 9785 on the photosynthetic competence of leaves, on the photochemical activity of isolated thylakoids and on the formation and spectral properties of chlorophyll-protein complexes were studied during a 72-h greening period of detached etiolated leaves of barley (Hordeum vulgare L. cv. Horpácsi kétsoros). It was established that i) the photosynthetic capacity of the leaves decreased considerably (by 80 and 90%, as determined by14CO2 fixation and fast fluorescence induction measurements, respectively); ii) the photochemical activity of isolated thylakoids from water to potassium ferricyanide and from dichlorophenol indophenol/ascorbate to methylviologen exhibited only slight reductions when expressed on a chlorophyll basis compared with the control; iii) the slow fluorescence induction curves of the treated leaves demonstrated the presence of a peculiar fluorescence component interrupting the quenching of fluorescence at around 1 min illumination; iv) a shortage of the chlorophyll-protein complex of photosystem I (CPI) occurred with a higher content of the monomer of the light harvesting complex in the thylakoids of treated leaves; and v) the fluorescence spectrum of the CPI band present in treated leaves indicates the destruction of the structural integrity of this complex during isolation from the membrane.
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Abbreviations
- Chl:
-
chlorophyll
- CPI, CPII:
-
chlorophyll-protein complexes of the reaction centres of PSI and PSII
- DCMU:
-
3-(3′,4′-dichlorophenyl)-1,1-dimethylurea
- DPIP:
-
2,6-dichlorophenol indophenol
- DPIPH2 :
-
chemically reduced form of DPIP
- F o :
-
fluorescence of constant yield
- F v :
-
fluorescence of variable yield
- F i ,F m :
-
mitial and maximum yield of fluorescence
- LHCP3 :
-
monomer of the light-harvesting complex
- LHCP2 and LHCP1 :
-
oligomers of the light-harvesting complex LHCP3
- PSI, PSII:
-
photosystems I, II
- SAN 9785:
-
4-chloro-5-(dimethylamino)-2-phenyl-3(2H)-pyridazinone, also known as BASF 13-338
- SDS-PAGE:
-
sodium dodecyl sulphate-polyacrylamide gel electrophoresis
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Laskay, G., Lehoczki, E., Dobi, A.L. et al. Photosynthetic characteristics of detached barley leaves during greening in the presence of SAN 9785. Planta 169, 123–129 (1986). https://doi.org/10.1007/BF01369783
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DOI: https://doi.org/10.1007/BF01369783