Abstract
Five types of anion-exchanger resin beads which had adsorbed indole-3-acetic acid (IAA) were tested as IAA donors. The rate of IAA-uptake by beads was a function of time and pH. The release was relatively steady during 6 h application on vertical maize roots. No IAA degradation occurred in the beads (Amberlite IRA 400 type) but 45.8% was metabolised in the roots during treatment. Beads loaded with IAA and placed on one side of the root (at 2.20±0.03 mm from the tip) induced a curvature towards and above the bead (23.3±1.1 degrees after 5.25 h application). In contrast, control beads (without IAA) did not change the axial growth rate. Applied IAA seemed to move differently from endogenous IAA. The use of resin beads loaded with IAA offers a technique to study the effects of local IAA application on intact growing roots.
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Abbreviations
- 3,3-DGA:
-
3,3 dimethyl-glutaric acid
- HPLC:
-
high-performance liquid chromatography
- IAA:
-
indole-3-acetic acid
- Ox-IAA:
-
oxindole-3-acetic acid
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Pilet, P.E., Meuwly, P. Local application of indole-3-acetic acid, by resin beads to intact growing maize roots. Planta 169, 16–22 (1986). https://doi.org/10.1007/BF01369770
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DOI: https://doi.org/10.1007/BF01369770