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Some regulatory aspects of [14C]methylamine influx intoPisum sativum L. cv. Feltham First seedlings

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Abstract

[14C]Methylamine influx intoPisum sativum L. cv. Feltham First seedlings showed Michaelis-Menten-type kinetics with apparentV max=49.2 μmol·g-1 FW·h-1 and apparentK m=0.51 mM. The competitive interactions between ammonium and methylamine were most obvious when biphasic kinetics were assumed with saturation of the first phase at ∼0.05 mM. The inhibitor constant for ammonium (K i)=0.027 mM. When [14C]methylamine was used in trace amounts with ammonium added as substrate, the influx of tracer showed Michaelis-Menten-type kinetics with apparentV max=3.46 μmol·g-1 FW·h-1 and apparentK m=0.15 mM. The initial rate of net ammonium uptake corresponded with that found when [14C]methylamine was used to ‘trace’ ammonium influx. The latter was also stimulated by high pHo and inhibited by nitrate. Ammonium pretreatment±methionine sulphoximine or glutamine pretreatment of the seedlings inhibited subsequent [14C]methylamine influx, while methylamine or asparagine pretreatment stimulated [14C]methylamine influx. There was also a stimulatory effect of prior inoculation withRhizobium. The results are discussed in terms of current models for the regulation of ammonium uptake in plants.

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References

  • Belton, P.S., Lee, R.B., Radeliffe, R.G. (1985) A14N nuclear magnetic resonance study of inorganic nitrogen metabolism in barley, maize and pea roots. J. Exp. Bot.36, 190–210

    Google Scholar 

  • Becking, J.H. (1956) On the mechanism of ammonium ion uptake by maize roots. Acta. Bot. Neerl.5, 2–79

    Google Scholar 

  • Boussiba, S., Dilling, W., Gibson, J. (1984) Methylamine transport inAnacystis nidulans. J. Bacteriol.160, 204–210

    PubMed  Google Scholar 

  • Boussiba, S., Gibson, J. (1985) The role of glutamine synthetase activity in ammonium and methylammonium transport inAnacystis nidulans R. 2. Febs. Letts.180, 13–16

    Google Scholar 

  • Cox, W.J., Reisenauer (1973) Growth and ion uptake by wheat supplied nitrogen as nitrate or ammonium or both. Plant Soil38, 303–380

    Google Scholar 

  • Deane-Drummond, C.E. (1984) Nitrate transport intoChara corallina cells using36ClO -3 as an analogue for nitrate. II. Comparison with14C methylamine fluxes at different pHo and NH +4 /NO -3 interactions. J. Exp. Bot.35, 1299–1308

    Google Scholar 

  • Deane-Drummond, C.E. (1985) A comparison of regulatory effects of chloride on nitrate uptake, and nitrate on chloride uptake intoPisum sativum seedlings. Physiol. Plant.66, 115–121

    Google Scholar 

  • Deane-Drummond, C.E., Chaffey, N.J. (1985) Characteristics of nitrate uptake into seedlings of pea (Pisum sativum L. c.v. Feltham First). Changes in net NO -3 uptake following inoculation withRhizobium and growth in low nitrate concentrations. Plant Cell Environ.8, 517–523

    Google Scholar 

  • Hackette, S.L., Skye, G.E., Burton, C., Segel, I.H. (1970) Characterization of an ammonium transport system in filamentous fungi with methylamonia [14C] as the substrate. J. Biol. Chem.245, 4241–4250

    PubMed  Google Scholar 

  • Jones, R.S., Patterson, R.P., Raper, C.D. Jr. (1981) The influence of temperature and nitrate on vegetative growth and nitrogen accumulation by nodulated soybeans. Plant Soil63, 333–334

    Google Scholar 

  • Joseph, R.A., van Hai, T., Lambert, J. (1975) Multiphasic uptake of ammonium by soybean roots. Physiol. Plant.34, 321–325

    Google Scholar 

  • Kleiner, D., Castorph, H. (1982) Inhibition of ammonium (methyl ammonium) transport inKlebsiella pneumoniae by glutamine and glutamine analogues. Febs. Letts.146, 201–203

    Google Scholar 

  • Lewis, O.A.M., Chadwick, S. (1983) A15N investigation into nitrogen assimilation in hydroponically grown barley (Hordeum vulgare c.v. Clipper) in response to nitrate, ammonium and mixed nitrate and ammonium nutrition. New Phytol.95, 635–646

    Google Scholar 

  • Lewis, O.A.M., Chadwick, S., Withers, J. (1983) The assimilation of ammonium by barley roots. Planta159, 483–486

    Google Scholar 

  • Marcus-Wyner, L., Rains, D.W. (1982) Simultaneous measurement of NH +4 absorption and N2 fixation byGlycine max L. Response to temperature, pH, and external nitrogen concentration. Plant Physiol.69, 460–464

    Google Scholar 

  • Marcus-Wyner, L., Rains, D.W. (1983) Patterns of ammonium absorption and acetylene reduction during soybean developmental growth. Physiol. Plant.59, 79–82

    Google Scholar 

  • McNaughton, G.S., Presland, M.R. (1983) Whole plant studies using radioactive 13-nitrogen. 1. Techniques for measuring the uptake and transport of nitrate and ammonium ions in hydroponically grownZea mays. J. Exp. Bot.34, 880–892

    Google Scholar 

  • Oghoghorie, G.G.O., Pate, J.S. (1971) The nitrate stress syndrome of the nodulated field pea (Pisum arvense L.) Plant Soil. (Spec. Vol.) 185–202

  • Smith, F.A., Walker, N.A. (1978) Entry of methylammonium and ammonium ions intoChara. J. Exp. Bot.29, 107–120

    Google Scholar 

  • Solózano, L. (1969) Determination of ammonia in natural waters by the phenylhypochloride method. Limnol. Ocean.14, 799–801

    Google Scholar 

  • Sprent, J.I., Minchin, F.R., Thomas, R.J. (1983) Environmental effect on the physiology of nodulation and nitrogen fixation. In: Davies, D.R., Jones, D.G. (eds) The physiology, genetics and nodulation of temperate legumes, pp. 269–317. Pitman, London

    Google Scholar 

  • Sprent, J.I., Thomas, R.J. (1984) Nitrogen nutrition of seedling grain legumes: some taxonomic, morphological and physiological constraints. Plant Cell Environ.7, 637–645

    Google Scholar 

  • Ullrich, W.R., Larsson, M., Larsson, C.M., Lesch, S., Novacky, A. (1984) Ammonium uptake inLemna gibba Gl., related membrane potential changes and inhibition of anion uptake. Physiol. Plant.61, 369–376

    Google Scholar 

  • Walker, N.A., Smith, F.A., Beilby, M.J. (1979) Amine uniport at the plasmalemma ofCharophyte cells. II. Ratio of matter to charge transported and permeability of free base. J. Membr. Biol.49, 283–296

    Google Scholar 

  • Warncke, D.D., Barber, S.A. (1975) Ammonium and nitrate uptake by corn (Zea mays) as influenced by nitrogen concentration and NH +4 /NO -3 . Agron. J.65, 950–953

    Google Scholar 

  • Wynch, R.D., Rains, D.W. (1978) Simultaneous measurement of nitrogen fixation estimated by acetylene-ethylene assay and nitrate absorption by soybeans. Plant Physiol.62, 433–448

    Google Scholar 

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  1. Botany Department, Science Laboratories, Durham University, DH1 3LE, Durham, UK

    C. E. Deane-Drummond

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Deane-Drummond, C.E. Some regulatory aspects of [14C]methylamine influx intoPisum sativum L. cv. Feltham First seedlings. Planta 169, 8–15 (1986). https://doi.org/10.1007/BF01369769

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  • DOI: https://doi.org/10.1007/BF01369769

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