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DNA profiling of bloodstains on linen pretreated with remedies used for cleaning and maintaining clothes

Summary

Linen was pretreated with 20 remedies for cleaning and maintaining clothes and 20 μl blood was applied on each sample and dried. Restriction enzyme digest of bloodstain DNA was irregularly inhibited by highly concentrated residues of 2 detergents and a stainremover and colour-brightener. An additional dialysis step to purify DNA (Gill 1987) reliably prevented disturbance. High molecular DNA was obtained in every case and bandshifts were not observed.

Zusammenfassung

Leinenstoff wurde mit 20 Mitteln, die zur Wäschereinigung und -pflege verwendet werden, in normaler und sehr hoher Konzentration behandelt, absichtlich unzureichend oder nicht gewaschen, getrocknet und mit 20 μL Blut versetz. Hohe Rückstandskonzentrationen von zwei Waschmitteln und eines Aufhellers führten unregelmäßig zu partieller und totaler Inhibition der Restriktionsspaltung. Durch einen Dialyseschritt bei der Reinigung der DNA (Gill 1987) konnten die Störungen zuverlässig verhindert werden. Hochmolekulare DNA konnte in jedem Fall gewonnen werden, Bandshifts traten nicht auf.

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Scheithauer, R., Weisser, H.J. DNA profiling of bloodstains on linen pretreated with remedies used for cleaning and maintaining clothes. Int J Leg Med 104, 273–275 (1991). https://doi.org/10.1007/BF01369584

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  • DOI: https://doi.org/10.1007/BF01369584

Key words

  • DNA profiling
  • Bloodstains
  • Stain carrier

Schlüsselwörter

  • DNA Profiling
  • Blutspuren
  • Spurenträger