Skip to main content
SpringerLink
Log in

Effect of chronically administered L-Dopa on Dopa/5HTP decarboxylase and tyrosine and tryptophan hydroxylases in cat brain

L'Activité des Hydroxylases de la Tyrosine et du Tryptophane et de la Décarboxylase de la Dopa et du 5-HTP du Cerveau de Chat à la Suite d'un Traitement Chronique à la L-Dopa

  • Published:
Journal of Neural Transmission Aims and scope Submit manuscript

Summary

The activity of tyrosine and tryptophan hydroxylases and of Dopa/ 5-HTP decarboxylase was measured in different structures of the brain of cats administered L-Dopa (100 mg/kg/day,per os) during several consecutive days. The activity of tyrosine hydroxylase which is unchanged after 4 and 7 days of treatment, respectively, is significantly decreased after 21 days of L-Dopa. The activity of tryptophan hydroxylase is normal after 4 days of L-Dopa but it is significantly decreased after 7 and 21 days of L-Dopa, respectively. The activity of decarboxylase is normal after 4 days of L-Dopa but it is significantly increased after 7 and 21 days of L-Dopa, respectively.

Résumé

L'activité des hydroxylases de la tyrosine et du tryptophane et de la décarboxylase de la Dopa et du 5-HTP a été mesurée au niveau de diverses structures du cerveau de chat à la suite d'un traitement chronique à la L-Dopa (100 mg/kg/jour,per os). Après 4 jours de traitement l'activité de ces enzymes demeure normale tandis qu'après 7 et 21 jours, l'activité de Phydroxylase du tryptophane est significativement diminuée et celle de la décarboxylase augmentée. Par ailleurs, l'activité de Phydroxylase de la tyrosine est significativement diminuée après 21 jours de traitement à la L-Dopa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahtee, L., D. F. Sharman, andM. Vogt: Acid metabolites of monoamines in avian brain. Brit. J. Pharm.38, 68–72 (1970).

    Google Scholar 

  • Anton, A. H., andD. F. Sayre: The distribution of dopamine and dopa in various animals and a method for their determination in diverse biological material. J. Pharmacol. Exp. Ther.145, 326–336 (1969).

    Google Scholar 

  • Ashcroft, G. W., D. Eccleston, andT. B. B. Crawford: 5-Hydroxyindole metabolism in rat brains. A study of intermediate metabolism using the technique of tryptophan loading. I. Methods. J. Neurochem.12, 483–492 (1965).

    Google Scholar 

  • Bartholini, G., andA. Pleischer: Cerebral accumulation and metabolism of C14-dopa after selective inhibition of peripheral decarboxylase. J. Pharmacol. Exp. Ther.161, 14–20 (1968).

    Google Scholar 

  • Bertler, A., B. Falck, Ch. Owman, andE. Rosengren: The localization of monoaminergic blood-brain barrier mechanisms. Pharmacol. Rev.18, 369–385 (1966).

    Google Scholar 

  • Christenson, J. G., W. Dairman, andS. Udenfriend: On the identity of Dopa decarboxylase and 5-hydroxytryptophan decarboxylase. Proc. Nat. Acad. Sci. (USA)69, 343–347 (1972).

    Google Scholar 

  • Cicero, T. J., L. G. Sharpe, E. Robins, andS. S. Grote: Regional distribution of tyrosine hydroxylase in rat brain. J. Neurochem.19, 2241–2243 (1972).

    Google Scholar 

  • Constantinidis, J., J. C. De La Torre, R. Tissot, andF. Geissbühler: La barrière capillaire pour la dopa dans le cerveau et les différents organes. Psychopharmacol. (Berl.)15, 75–87 (1969).

    Google Scholar 

  • Dairman, W., J. G. Christenson, andS. Udenfriend: Decrease in liver aromatic-L-amino-acid decarboxylase produced by chronic administration of L-Dopa. Proc. Nat. Acad. Sci. (USA)68, 2117–2120 (1971).

    Google Scholar 

  • Dairman, W., J. G. Christenson, andS. Udenfriend: Changes in tyrosine hydroxylase and dopa decarboxylase induced by pharmacological agents. Pharmacol. Rev.24, 269–290 (1972).

    Google Scholar 

  • Dairman, W., andS. Udenfriend: Decrease in adrenal tyrosine hydroxylase and increase in norepinephrine synthesis in rats given L-Dopa. Science171, 1022–1024 (1971).

    Google Scholar 

  • Dairman, W., andS. Udenfriend: Studies on the mechanism of the L-3, 4-dihydroxyphenylalanine induced decrease in tyrosine hydroxylase activity. Mol. Pharmacol.8, 293–299 (1972).

    Google Scholar 

  • Davidson, L., K. Lloyd, I. Dankova, andO. Hornykiewicz: L-Dopa treatment in Parkinson's disease: Effect on dopamine and related substances in discrete brain regions. Experientia27, 1048–1049 (1971).

    Google Scholar 

  • Duvoisin, R. C., M. Yahr, andL. D. Côté: Pyridoxine reversal of L-Dopa effects in parkinsonism. Trans. Amer. Neurol. Ass.94, 81–84 (1969).

    Google Scholar 

  • Freedland, R. A., I. M. Wadznski, andH. A. Waisman: The enzymatic hydroxylation of tryptophan. Biochem. Biophys. Res. Commun.5, 94–103 (1961).

    Google Scholar 

  • Goldstein, M., andR. Freukel: Inhibition of serotonin synthesis by Dopa and other catechols. Nature New Biol.233, 179–180 (1971).

    Google Scholar 

  • Goldstein, M., T. H. Joh, andH. Gang: Some aspects of the biosynthesis of catecholamines in adrenal glands and in pheochromocytoma and neuroblastoma tremors. 7th Int. Cong. Clin. Chem.3, 78–84 (1970).

    Google Scholar 

  • Grahame-Smith, D. F.: The biosynthesis of 5-hydroxy-tryptamine in brain. Biochem. J.105, 351–360 (1967).

    Google Scholar 

  • Kitsikis, Anne, andAndrée G. Roberge: Behavioural and biochemical effects of L-Dopa in cats. Israel J. Med. Sci. (1972, in press).

  • Kuczenski, R. T., andA. J. Mandell: Regulatory properties of soluble and paniculate rat brain tyrosine hydroxylase. J. Biol. Chem.247, 3114–3122 (1972).

    Google Scholar 

  • Kuntzman, R., P. Shore, D. Bogdanski, andB. B. Brodie: Microanalytical procedures for fluorometric assay of brain Dopa-5-HTP decarboxylase, norepinephrine and serotonin, and a detailed mapping of decarboxylase activity in brain. J. Neurochem.6, 226–232 (1961).

    Google Scholar 

  • Lancaster, G. A., andT. L. Sourkes: Purification and properties of dopa decarboxylase of hog kidney. Can. J. Biochem.50, 7, 791–797 (1972).

    Google Scholar 

  • Langelier, P., A. Parent, andL. J. Poirier: Decarboxylase activity of the brain capillary walls and parenchyma in the rat, cat and monkey. Brain Res.45, 622–629 (1972).

    Google Scholar 

  • Leon, A. S., H. E. Spiegel, G. Thomas, Ira Ross, andW. B. Abrams: Effects of pyridoxine on adsorption, metabolism and clinical activity of levodopa in Parkinson's disease. Clin. Pharmacol. and Ther.12, 294–302 (1971).

    Google Scholar 

  • Lewitt, M., S. Spector, A. Sjoerdsma, andS. Udenfriend: Elucidation of the rate-limiting step in norepinephrine biosynthesis using the perfused guinea pig heart. J. Pharmacol. Exp. Ther.148, 1–8 (1965).

    Google Scholar 

  • Lison, L.: Statistique appliquée à la biologie expérimentale. Paris: Gauthier Villars. 1958.

    Google Scholar 

  • Lovenberg, W., E. Jequier, andA. Sjoerdsma: Tryptophan hydroxylation: measurement in pineal gland, brainstem and carcinoid tumor. Science (New York)155, 217–219 (1967).

    Google Scholar 

  • Lovenberg, W., H. Weissbach, andS. Udenfriend: Aromatic L-amino acid decarboxylases. J. Biol. Chem.237, 89–93 (1962).

    Google Scholar 

  • Lowry, O. H., H. J. Rosebrough, A. L. Farr, andR. J. Randall: Protein measurement with the folin phenol reagent. J. Biol. Chem.193, 265–275 (1951).

    Google Scholar 

  • McGeer, E. G., S. Gibson, J. A. Wada, andP. L. McGeer: Distribution of tyrosine hydroxylase activity in adult and developing brain. Can. J. Bioch.45, 1943–1952 (1967).

    Google Scholar 

  • McGeer, E. G., S. Gibson, andP. L. McGeer: Some characteristics of brain tyrosine hydroxylase. Can. J. Bioch.45, 1557–1563 (1967).

    Google Scholar 

  • Nagatsu, T., M. Lewitt, andS. Udenfriend: Tyrosine hydroxylase-the initial step in NE biosynthesis. J. Biol. Chem.239, 2910–2917 (1964).

    Google Scholar 

  • Nagatsu, T., andT. Yamamoto: Fluorescence assay of tyrosine hydroxylase activity in tissue homogenate. Experientia24, 1183–1184 (1968).

    Google Scholar 

  • Peters, D. A. V., P. L. McGeer, andE. G. McGeer: The distribution of tryptophan hydroxylase in cat brain. J. Neurochem.15, 1431–1435 (1968).

    Google Scholar 

  • Schott, H. F., andW. G. Clark: Dopa decarboxylase inhibition through the interaction of coenzyme and substrate. J. Biol. Chem.196, 449–462 (1952).

    Google Scholar 

  • Sourkes, T. L.: Dopa decarboxylase: Substrates, coenzymes, inhibitors. Pharmacol. Rev.18, 53–60 (1966).

    Google Scholar 

  • Sourkes, T. L.: Influence of specifie nutrients on catecholamine synthesis and metabolism. Pharmacol. Rev.24, 349–359 (1972).

    Google Scholar 

  • Tate, S. S., R. Sweet, F. H. McDowell, andA. Meister: Decrease of the 3, 4-dihydroxyphenylalanine (Dopa) decarboxylase activities in human erythrocytes and mouse tissues after administration of Dopa. Proc. Nat. Acad. Sci. (USA)68, 2121–2134 (1971).

    Google Scholar 

  • Udenfriend, S.: Tyrosine hydroxylase. Pharmacol. Rev.18, 43–51 (1966).

    Google Scholar 

  • Udenfriend, S., P. Zaltzman-Nirenberg, andT. Nagatsu: Inhibitors of purified beef adrenal tyrosine hydroxylase. Biochem. Pharmacol.14, 837–845 (1965).

    Google Scholar 

  • Waalkes, T. P., andS. Udenfriend: A fluorometric method for the estimation of tyrosine in plasma and tissues. J. Lab. Clin. Med.50, 733–736 (1957).

    Google Scholar 

  • Waymire, J. C., N. Weiner, F. H. Schneider, M. Goldstein, andL. S. Freedman: Tyrosine hydroxylase in human adrenal and pheochromocytoma: localization, kinetics and catecholamine inhibition. J. Clin. Invest.57, 1798–1804 (1972).

    Google Scholar 

Download references

Author information

Author notes

  1. Andrée G. Roberge

    Present address: Laboratoires de Neurobiologie, Faculté de Médecine, Université Laval, Québec, Canada

Authors and Affiliations

  1. Laboratoires de Neurobiologie, Faculté de Médecine, Université Laval, Québec, Canada

    L. J. Pokier

Authors
  1. Andrée G. Roberge
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. J. Pokier
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roberge, A.G., Pokier, L.J. Effect of chronically administered L-Dopa on Dopa/5HTP decarboxylase and tyrosine and tryptophan hydroxylases in cat brain. J. Neural Transmission 34, 171–185 (1973). https://doi.org/10.1007/BF01367507

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01367507

Keywords

Search

Navigation