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Control of secretory granule interaction and exocytosis in pancreatic cells

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Abstract

Application of the laser-based technique of photon correlation spectroscopy to anin vitro study of the ionic stability and interaction kinetics of zymogen granules isolated from rat exocrine pancreas is described here. In addition the separation from pancreatic acinar cell cytosol of a factor which stabilizes isolated zymogen granules and inhibits cation-induced granule aggregation is outlined. The basis of this action and the significance of the cytosolic inhibitory factor in the regulation of granule mobility and exocytosisin vivo is discussed.

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References

  • Burwen, S. J. and Rothman, S. S. (1972). Zymogen granules: osmotic properties, interactions with ions, and some structural implications.Am. J. Physiol. 222:1177–1181.

    Google Scholar 

  • Hokin, L. E. (1955). Isolation of the zymogen granules of dog pancreas and a study of their properties.Biochim. Biophys. Acta 18:379–388.

    Google Scholar 

  • Matthews, E. K. and O'Connor, M. D. L. (1978). Photon correlation spectroscopy and Laser-Doppler anemometry; a new method for determining secretory particle electrophoretic properties.J. Physiol. 278:1P.

    Google Scholar 

  • O'Connor, M. D. L. and Matthews, E. K. (1980). Membrane surface interactions involved in insulin secretion.Horm. Metab. Res. 12:Suppl. 10, 149–153.

    Google Scholar 

  • O'Doherty, J. and Stark, R. J. (1982). Stimulation of pancreatic acinar secretion: increases in cytosolic calcium and sodium.Am. J. Physiol. 242:G513-G521.

    Google Scholar 

  • Palade G. E. (1975). Intracellular aspects of the process of protein synthesis.Science 189:347–358.

    Google Scholar 

  • Poulsen, J. H. and Oakley, B. (1979). Intracellular potassium ion activity in resting and stimulated mouse pancreas and submandibular gland.Proc. R. Soc. Lond. Ser. B,294:90–104.

    Google Scholar 

  • Priessler, M. and Williams, J. A. (1981). Pancreatic acinar cell function: measurement of intracellular ions and pH and their relation to secretion.J. Physiol. 321:437–448.

    Google Scholar 

  • Rogers, J., Matthews, E. K. and McKay, D. B. (1987). Effects of a cytosolic protein on the interaction of rat pancreatic zymogen granulesin vitro.Biochim. Biophys. Acta 897:217–228.

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, University of Cambridge, Hills Road, CB2 2QD, Cambridge

    E. K. Matthews, Jane Rogers & D. B. McKay

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  1. E. K. Matthews
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  2. Jane Rogers
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  3. D. B. McKay
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Matthews, E.K., Rogers, J. & McKay, D.B. Control of secretory granule interaction and exocytosis in pancreatic cells. Biosci Rep 7, 435–442 (1987). https://doi.org/10.1007/BF01362506

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  • DOI: https://doi.org/10.1007/BF01362506

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