Summary
Dictyosome ultrastructure changes during the cell cycle in onion root meristematic cells. Changes were mainly related to cisternae and intercisternal spaces morphology. Taking each dictyosome to be composed of three different regions (CIS, medial, and TRANS), several quantitative changes were detected in some of the compartments. Many of the planimetric parameters evaluated showed higher values in medical cisternae, while CIS and TRANS remained nearly constant. We have also found an increased activity of dictyosomes, as indicated by increase in the volume fraction of vesicular attached structures. This reaches maximum values at ana-telophase in coincidence with the onset and progression of cytokinesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- A:
-
anaphase
- Ac:
-
mean area occupied by cisternae per stack section
- C:
-
CIS
- CCS:
-
cell cycle stage
- DA :
-
mean total dictyosome area
- ISA :
-
mean area occupied by intercisternal spaces per stack section
- M:
-
metaphase
- N:
-
mean number of cisternae per stack
- P:
-
prophase
- S.E.:
-
standard error
- T:
-
telophase
- T:
-
TRANS
- Vv :
-
volume density
References
Cunningham WP, Morré DJ, Mollenhauer HH (1966) Structure of isolated plant Golgi apparatus revealed by negative stain. J Cell Biol 28: 169–179
Dunphy WG, Brands R, Rothman JE (1985) Attachment of terminal N-acetylglucosamine to asparagine-linked oligosaccharides occurs in central cisternae of the Golgi stack. Cell 40: 463–472
—,Rothman JE (1985) Compartmental organization of the Golgi stack. Cell 42: 13–21
Giménez-Martín G, De La Torre C, López-Sáez JF (1977) Cell division in higher plants. In:Rost TL, Gifford EM (eds) Mechanisms and control of cell division. Dowden, Hutchinson and Ross Inc., Stroudsburg, pp 261–307
García-Herdugo G,González-Reyes JA,Gracía-Navarro F,Navas P (1988) Growth kinetics of the Golgi apparatus during the cell cycle in onion root meristems. Planta (in press)
Gonzalez-Reyes JA,Gracia-Navarro F,García-Herdugo G,Hidalgo A,Navas P (1988) Differential morphometric values induced in Golgi apparatus of higher plant cells by aldehyde and permanganate fixations. J Electron Microsc Tech (in press)
Gunning BES (1982) The cytokinetic apparatus: its development and spatial regulation. In:Lloyd CW (ed) The cytoskeleton in plant growth and development. Academic Press, London, pp 229–295
—,Steer MW (1975) Ultrastructure and the biology of plant cells. Arnold, London
Hepler PK (1982) Endoplasmic reticulum in the formation of cell plate and plasmodesmata. Protoplasma 111: 121–133
—,Wolniak SM (1984) Membranes in the mitotic apparatus: their structure and function. Int Rev Cytol 90: 169–238
Kristen U (1978) Ultrastructure and a possible function of the intercisternal elements in dictyosomes. Planta 138: 29–33
López-Sáez JF, Navarrete HH, Becerra J (1981) Regulation of cell division in meristems. Bol Soc Brot 53: 1315–1330
Mollenhauer HH, Mollenhauer BA (1978) Changes in the secretory activity of the Golgi apparatus during the cell cycle in root tips of maize (Zea mays). Planta 138: 113–118
—,Morré DJ (1975) A possible role for intercisternal elements in the formation of secretory vesicles in plant Golgi apparatus. J Cell Sci 19: 231–237
— — (1978) Structural differences contrast plant and animal Golgi apparatus. J Cell Sci 32: 357–362
— — (1980) The Golgi apparatus. In:Stumpf PK, Conn EE (eds) Biochemistry of plants. A comphrehensive treatise, vol 1. Academic Press, New York, pp 437–488
Morré DJ (1977) Membrane differentiation and the control of secretion: a comparison of plant and animal Golgi apparatus. In: International cell biology 1976–1977. Springer, Berlin, pp293–303
Navas P, Minnifield N, Sun I, Morré DJ (1986) NADP phosphatase as a marker in free-flow electrophoretic separations for cisternae of the Golgi apparatus midregion. Biochim Biophys Acta 881: 1–9
Pavelka M, Ellinger A (1986) RCA I-binding patterns of the Golgi apparatus. J Cell Biol 41: 270–278
Paweletz N (1981) Membranes in the mitotic apparatus. Cell Biol Int Rep 5: 323–336
Reynolds ES (1962) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17: 208
Robinson DG, Kristen U (1982) Membrane flow via the Golgi apparatus of higher plant cells. Int Rev Cytol 77: 89–127
Sans J, Giménez-Martín G, De La Torre C (1980) Onset of cell proliferation in dormant cells ofAllium cepa L bulbs: kinetic analysis. Biol Cell 38: 95–104
Taatjes DT, Roth J (1986) The trans-tubular network of the hepatocyte Golgi apparatus is part of the secretory pathway. Eur J Cell Biol 42: 344–350
Weibel ER (1979) Practical methods for biological morphometry. In:Weibel ER (ed) Stereological methods, vol 1. Academic Press, London, pp 101–159
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gonzalez-Reyes, J.A., Gracía-Navarro, F., Garcia-Herdugo, G. et al. Changes of dictyosome ultrastructure during the cell cycle in onion root meristematic cells. A morphometric and stereological study. Protoplasma 146, 35–40 (1988). https://doi.org/10.1007/BF01354293
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01354293