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A cytokinin-sensitive mutant of the moss,Physcomitrella patens, defective in chloroplast division

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Summary

An X-ray induced mutant (PC22) of the moss,Physcomitrella patens was analysed with respect to its morphology, physiology and suitability for microculture techniques. The mutant protonemata are defective in bud formation and in chloroplast division. As a consequence of the latter, giant chloroplasts are formed which disturb the development of the phragmoplast, the formation of regular cross walls, and cell division. Abnormal cross walls are rich in callose. The actin cytoskeleton was found to be less regularly developed in the mutant than in the wild type. Three-dimensional analysis of the microtubular arrangement with confocal laser scan microscopy demonstrates a close association between spindle- or phragmoplast- and “interphase”-microtubules. The deficiencies in chloroplast division and in bud formation can partly be compensated for by exogeneously applied cytokinin. The suitability of this particular developmental mutant for further studies was shown by regeneration of protoplasts in microculture and microinjection of the fluorochrome Lucifer yellow into the chloroplast.

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Abbreviations

CLSM:

confocal laser scan microscope

DAPI:

diamidinophenyl indole

DiOC:

3,3′-dihexyloxacarbocyanine iodide

EGTA:

ethylene glycol-bis-(β-amino-ethylether-N′,N′,N′,N′-tetraacetic acid

i6Ade:

N6-(2-isopentenyladenine)

PIPES:

piperazine-N, N′-bis-2-ethanesulfonic acid

ptDNA:

chloroplast DNA

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Authors and Affiliations

  1. Institut für Allgemeine Botanik, Universität Hamburg, Hamburg

    W. O. Abel, J. R. Marienfeld & R. Reski

  2. Zellenlehre, Universität Heidelberg, Heidelberg

    W. Knebel, H. Quader & E. Schnepf

  3. Botanisches Institut, Universität München, München

    H. -U. Koop & B. Spörlein

Authors
  1. W. O. Abel
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  2. W. Knebel
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  3. H. -U. Koop
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  4. J. R. Marienfeld
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  5. H. Quader
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  6. R. Reski
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  7. E. Schnepf
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  8. B. Spörlein
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Devoted to the memory of Prof. Dr. O. Kiermayer, our colleague and friend.

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Abel, W.O., Knebel, W., Koop, H.U. et al. A cytokinin-sensitive mutant of the moss,Physcomitrella patens, defective in chloroplast division. Protoplasma 152, 1–13 (1989). https://doi.org/10.1007/BF01354234

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  • DOI: https://doi.org/10.1007/BF01354234

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