Summary
The mammalian centrosome monoclonal antibody MPM-13 recognized component(s) of the well defined MTOC basal-body root complex in the green plantChlamydomonas. The antibody reaction coincided in location with the basal-body root complex and the cruciate nature of the staining pattern corresponded to the configuration of the root microtubules. During mitosis the behaviour of MPM-13 stained material mirrored the duplication, separation and migration to the spindle poles of the basal body-root complex. It is suggested that conserved MTOC components were recognized and that these may have retained a similar, perhaps universal, function in microtubule organization.
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Abbreviations
- BSA:
-
bovine serum albumin
- DAPI:
-
4,6-diamidine-2-phenylindole dihydrochloride
- mt:
-
mating type
- MT:
-
microtubule
- MTOC:
-
microtubule organizing centre
- PFA:
-
paraformaldehyde
- PBS:
-
phosphate buffered saline
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Harper, J.D.I., Rao, P.N. & John, P.C.L. A monoclonal antibody to animal centrosomes recognizes components of the basal-body root complex inChlamydomonas . Protoplasma 153, 208–211 (1990). https://doi.org/10.1007/BF01354006
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DOI: https://doi.org/10.1007/BF01354006