Summary
Localization of acid phosphatase in mammary glands of lactating rats was studied by both biochemical and cytochemical methods. Cytochemically, acid phosphatase activity was detected by using lead citrate as the capture agent for the inorganic phosphate released from p-nitrophenyl phosphate. The activity was predominantly localized in the lumina of the endomembrane system and in the milk that had been secreted into the alveolar lumen. Biochemically, acid phosphatase was present in all the subcellular fractions with higher activities in the membrane-associated fractions. The localization of tartrate-resistant acid phosphatases within the endomembrane system of fully lactating rat mammary tissue suggests a possible role for these enzymes in milk secretory processes.
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Abbreviations
- ASMX:
-
3-hydroxy-2-naphthoic acid 2,4-dimethylanilide
- DMSO:
-
dimethylsulfoxide
- DTT:
-
dithiothreitol
- EDTA:
-
ethylenedinitrilo tetra-acetic acid
- FGM:
-
fat globule membranes
- MES:
-
2-(N-morpholino) ethanesulfonic acid
- PCMB:
-
p-chloromercuribenzoate
- p-NPP:
-
p-nitrophenyl phosphate
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Leung, C.T., Maleeff, B.E., Wickham, E.D. et al. Localization of acid phosphatase in lactating rat mammary glands. Protoplasma 153, 149–156 (1990). https://doi.org/10.1007/BF01353999
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DOI: https://doi.org/10.1007/BF01353999