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Serologic specificity of antibodies to herpes simplex virus glycoprotein B present in human sera Analysis by transient expression of glycoprotein B-derivatives

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Summary

Glycoprotein B (gB) is an essential glycoprotein of herpes simplex virus (HSV) and a major target for cellular and humoral immune response in the infected host. In the present study, we have analyzed the pattern of reactivity of a panel of 23 HSV-seropositive patient sera using as test antigens gB derivatives made in COS cells in a transient expression assay. Our results show that nearly all the sera tested, reacted with wild-type gB or tgB (772) (that lacks 102 amino acids cytoplasmic domain). However, when tgB (477 amino acids) or gBdl (an inframe deletion between amino acids 477–772) were used as test antigens only 12 out of 23 sera tested positive. Further analysis using competition assays revealed that these sera can be classified into at least two groups: (i) that contain gB-reactive antibodies reactive to intact gB or tgB (772); (ii) that contain antibodies that recognize all forms of gB-derivatives tested. The results presented here underscore the potential limitations in using certain truncated forms of gB as antigens for subunit vaccine or in the serodiagnosis of HSV infection.

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Authors and Affiliations

  1. Viral Carcinogenesis Laboratory, Linus Pauling Institute, Palo Alto, USA

    Mir A. Ali

  2. California Department of Health Services, Viral and Rickettsial Disease Laboratory, Berkeley, California, USA

    B. Forghani

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  1. Mir A. Ali
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  2. B. Forghani
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Ali, M.A., Forghani, B. Serologic specificity of antibodies to herpes simplex virus glycoprotein B present in human sera Analysis by transient expression of glycoprotein B-derivatives. Archives of Virology 112, 129–138 (1990). https://doi.org/10.1007/BF01348990

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  • DOI: https://doi.org/10.1007/BF01348990

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