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Circular forms of viral DNA in Marek's disease virus-transformed lymphoblastoid cells

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Summary

The state of viral DNA in three Marek's disease virus (MDV)-transformed chicken cell lines (MDCC-MSB-1, MDCC-HP-2, MDCC-RP-1) was investigated by CsCl-density gradient centrifugation, and sedimentation analysis in neutral glycerol gradients. Each cell line contained MDV DNA integrated into the host cell genome. Additionally, free viral DNA could be identified in MDCC-MSB-1 and MDCC-HP-2 cells, sedimenting at about 100 S, and banding at the position of circular DNA in CsCl-ethidiumbromide gradients. Thus, MDV DNA with properties of circular plasmid DNA could be demonstrated in 2 virus-transformed cell lines. The significance of circular plasmid forms of viral DNA remains yet to be clarified, since these are apparently not regularly found in MDV-transformed cells.

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References

  1. Adams, A., Lindahl, T., Klein, G.: Linear association between cellular DNA and Epstein-Barr virus DNA in human lymphoblastoid cell line. Proc. Nat. Acad.70, 2888–2892 (1973).

    Google Scholar 

  2. Adams, A.: The state of the virus genome in transformed cells and its relationship to host cell DNA. In:Epstein, M. A., Achong, B. G. (eds.), The Epstein Barr Virus, 155–178. Berlin-Heidelberg-New York: Springer 1979.

    Google Scholar 

  3. Akiyama, Y., Kato, S.: Continuous cell culture from lymphoma of Marek's disease. Biken's J.16, 177–179 (1973).

    Google Scholar 

  4. Ben-Porat, T., Tokazewski, S. A.: Replication of Herpesvirus DNA. II. Sedimentation characteristics of newly synthesized DNA. Virology79, 292–301 (1977).

    Google Scholar 

  5. Gussander, E., Adams, A.: New intracellular states of Epstein-Barr virus DNA in the Burkitt's-lymphoma-derived Raji cell line. In:Essex, M., Todaro. G., Zur Hausen, H. (eds.), Viruses in naturally occurring cancers, CHS Lab.7, 23–34 (1980).

    Google Scholar 

  6. Kaschka-Dierich, C., Nazerian, K., Thomssen, R.: Intracellular state of Marek's disease virus DNA in two tomour-derived chicken cell lines. J. gen. Virol.44, 271–280 (1979).

    Google Scholar 

  7. Kaschka-Dierich, C., Thomssen, R.: Studies on the temperature-dependent DNA replication of the Herpesvirus of the turkey in chicken embryo fibroblasts. J. gen. Virol.45, 253–261 (1979).

    Google Scholar 

  8. Lee, L. F., Kieff, E. D., Bachenheimer, S. L., Roizman, B., Spear, P. G., Burmester, B. R., Nazerian, K.: Size and composition of Marek's disease virus deoxyribonucleic acid. J. Virol.7, 289–294 (1971).

    Google Scholar 

  9. Lee, L. F., Nazerian, K., Boezi, J. A.: Marek's disease virus DNA in a chicken lymphoblastoid cell line (MSB-1) and in virus-induced tumours. In:De Thé, G., Epstein, M. A., Zur Hausen, H. (eds.), Scientific Publications, Oncogenesis and Herpesviruses, Vol. II, 199–204. Lyon: International Agency for Research on Cancer 1975.

    Google Scholar 

  10. Lindahl, T., Adams, A., Bjursell, G., Bornkamm, G. W., Kaschka-Dierich, C., Jehn, U.: Covalently closed circular duplex DNA of Epstein-Barr virus in a human lymphoid cell line. J. Mol. Biol.102, 511–530 (1976).

    Google Scholar 

  11. Nazerian, K., Lindahl, T., Klein, G., Lee, L. F.: Deoxyribonucleic acid of Marek's disease virus in virus-induced tumours. J. Virol.12, 841–846 (1973).

    Google Scholar 

  12. Nazerian, K., Lee, L. F.: Deoxyribonucleic acid of Marek's diseases virus in a lymphoblastoid cell line from Marek's disease tumours. J. gen. Virol.25, 317–321 (1974).

    Google Scholar 

  13. Nazerian, K., Stephens, E. A., Sharma, J. M., Lee, L. F., Lailitis, M., Witter, R. L.: A nonproducer T lymphoblastoid cell line from Marek's disease transplantable tumour (JMV). Avian Dis.21, 69–76 (1977).

    Google Scholar 

  14. Nazerian, K.: Marek's disease lymphoma of chicken and its causative herpesvirus. Biochim. et Biophys. Acta560, 375–395 (1979).

    Google Scholar 

  15. Powell, P. C., Payne, L. N., Frazier, J. A., Rennie, M.: T lymphoblastoid cell lines from Marek's disease lymphomas. Nature251, 79–80 (1974).

    Google Scholar 

  16. Tanaka, A., Silver, S., Nonoyama, M.: Biochemical evidence of the nonintegrated status of Marek's disease virus DNA in virus-transformed lymphoblastoid cells of chicken. Virology88, 19–24 (1978).

    Google Scholar 

  17. Werner, F.-J., Bornkamm, G. W., Fleckenstein, B.: Episomal viral DNA in a Herpesvirus saimiri-transformed lymphoid cell line. J. Virol.22, 794–803 (1977).

    Google Scholar 

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Authors and Affiliations

  1. Federal Research Institute for Animal Virus Diseases, Tübingen, Germany

    H. -J. Rziha & B. Bauer

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  1. H. -J. Rziha
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  2. B. Bauer
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Rziha, H.J., Bauer, B. Circular forms of viral DNA in Marek's disease virus-transformed lymphoblastoid cells. Archives of Virology 72, 211–216 (1982). https://doi.org/10.1007/BF01348966

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  • DOI: https://doi.org/10.1007/BF01348966

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