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Increased detection and yield of adenovirus in caffeine-stabilised KB cell monolayers

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Summary

Viable monolayers of KB cells were maintained without passage for up to one year by adding 2 × 10−3 m caffeine to the medium, while untreated monolayers degenerated after 2 weeks. When adenoviruses type 2 and 17 were titrated on caffeine-stabilised KB cells, the late breakthrough of cytopathic effect at terminal dilutions (up to 40 days after inoculation) resulted in titre determinations up to 100-fold higher than those measured before spontaneous degeneration in untreated cells. Yields of adenovirus type 2 in caffeine-treated KB cultures infected at different multiplicities and harvested at different intervals were equal to, or up to 100-fold higher than those obtained in untreated cultures.

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Authors and Affiliations

  1. Department of Microbiology, Brussels, Belgium

    G. Lion, Muriel Mombaerts-Servais & R. F. Mouton

  2. Institute of Hygiene and Epidemiology, Brussels, Belgium

    G. Lion, Muriel Mombaerts-Servais & R. F. Mouton

Authors
  1. G. Lion
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  2. Muriel Mombaerts-Servais
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  3. R. F. Mouton
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Additional information

With 2 Figures

Deceased.

Head: Dr. E. van Oye.

Director: Dr. A. Lafontaine.

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Lion, G., Mombaerts-Servais, M. & Mouton, R.F. Increased detection and yield of adenovirus in caffeine-stabilised KB cell monolayers. Archives of Virology 52, 263–268 (1976). https://doi.org/10.1007/BF01348024

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  • DOI: https://doi.org/10.1007/BF01348024

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