Summary
A comparison of the coat protein patterns of the wild types of the related phages T3 and T7 on SDS polyacrylamide gel electrophoresis was carried out. After infection of the nonpermissive host with T7 amber mutants in genes 7, 11, 12, 13 or 17 and T3 amber mutants in genes 11, 12, 13 and 17 respectively, noninfectious, DNA containing particles were produced. The protein pattern, as well as electron microscopy of defective particles of T3 and T7 led to the conclusion that the proteins specified by genes 11, 12, 13 and 17 are tail proteins whereas the proteins coded by genes 7, 8, 10, 14, 15 and 16 enter the head structure. The “serum blocking protein” (gene 17 product) seems to play a different role in the assembly of T3 and T7 tails, since T3 particles defective in gene 17 did not show any tail structure connected with the head whereas T7 particles defective in gene 17 had a tail which looked different from that of the wildtype.
Treatment of wildtype particles with ammonium nitrate or sodium pyrophosphate led to morphologically abberrant forms which had partially or completely lost the hexagonal head structure. After treatment with ammonium nitrate balllike structures were obtained, both for T3 and T7. However, in the case of T3 these abberrant forms contained the proteins specified by genes 7, 8 and 10 whereas those derived from T7 contained only the proteins specified by genes 8 and 10.
Sodium pyrophosphate treatment of T3 and T7 wildtype particles led to a release of the phage tails. The isolated tails were examined by electron microscopy thus revealing for the first time a detailed substructure of the T3 and T7 phage tails.
In order to find out more about the tail proteins, adsorption experiments with isolated bacterial cell walls were carried out.
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References
Adolph, K. W., Haselkorn, R.: Comparison of the structures of blue-green algal viruses LPP-1m and LPP-2 and bacteriophage T7. Virology47, 701–710 (1972).
Beier, H., Hausmann, R.: The genetic map of bacteriophage T3. J. Virol.12, 417–419 (1973).
Bradley, D. E.: Ultrastructure of bacteriophages and bacteriocins. Bact. Rev.31, 230–314 (1967).
Caro, L. G.: The molecular weight of Lambda DNA. Virology1, 226–236 (1966).
Casjens, S., Hohn, T., Kaiser, A. D.: Morphological proteins of phage Lambda: Identification of the major head protein as the product of gene E. Virology42, 496–507 (1970).
Davis, R., Hyman, R.: A study in evolution: The DNA sequence homology between coli phages T7 and T3. J. mol. Biol.62, 287–301 (1971).
Hausmann, R., Gomez, B.: Amber mutants of bacteriophages T3 and T7 defective in phage-directed deoxyribonucleic acid synthesis. J. Virol.1, 779–792 (1967).
Hausmann, R., Härle, E.: Expression of the Genomes of the related bacteriophages T3 and T7.Broda, E., Locker, A., Springer, H. (eds.), First Eur. Biophys. Cong., Baden, Austria. Wiener Med. Akad.: Lederer Verl. 1971.
Hausmann, R., Larue, K.: Variations in sedimentation patterns among deoxyribonucleic acid synthesized after infection ofEschrichia coli by different amber mutants of bacteriophage T7. J. Virol.3, 278–281 (1969).
Israel, J. V., Anderson, T. F., Levine, M.:In vitro morphogenesis of phage P22 from heads and base-plate parts. Proc. Nat. Acad. Sci. U.S.A.57, 284–291 (1966).
Issinger, O.-G.: Genetische Kontrolle der Proteinsynthese heptoider Phagen. Inaugural-Dissertation, Universität Freiburg, Germany (1973).
Issinger, O.-G., Beier, H., Hausmann, R.:In vivo andin vitro “phenotypic mixing” with amber mutants of phages T3 and T7. Mol. Gen. Genet.122, 81–88 (1973).
Issinger, O.-G., Hausmann, R.: Synthesis of bacteriophage-coded gene products during infection ofE. coli with amber mutants of T3 and T7 defective in gene 1. J. Virol.11, 465–475 (1973).
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227, 680–685 (1970).
Maizel, J. V., Jr.: Polyacrylamide gel electrophoresis of viral proteins. Methods. Virol.5, 179–190 (1971).
Misra, D. N., Sinha, R. K., das Gupta, N. N.: Molecular weight of DNA from Coliphage T7 by electron microscopy. Virology39, 183–187 (1969).
Parkinson, J. S., Huskey, R. J.: Deletion mutants of bacteriophage Lambda. J. mol. Biol.56, 369–381 (1971).
Reid, M. S., Bieleski, R. L.: A simple apparatus for verticle flatsheet polyacrylamide gel electrophoresis. Anal. Biochem.22, 374–381 (1968).
Ritchie, D. A., Malcolm, F. E.: Heat-stable and density mutants of phages T1, T3 and T7. J. gen. Virol.9, 35–43 (1971).
Studier, F. W.: Bacteriophage T7. Science176, 367–376 (1972).
Weber, K., Osborne, N.: The reliability of molecular weight determination by dodecylsulfate-polyacrylamide gel electrophoresis. J. biol. Chem.244, 4406–4412 (1969).
Yamamoto, K. R., Alberts, B. M., Benzinger, R., Lawthorne, L., Treiber, G.: Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale purification. Virology40, 734–744 (1970).
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Issinger, O.G., Falk, H. Comparative studies on the structure proteins of T3 and T7 phages. Archives of Virology 52, 217–231 (1976). https://doi.org/10.1007/BF01348019
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DOI: https://doi.org/10.1007/BF01348019