Abstract
Roots of ten-days-old seedlings obtained from a maize hybrid grown in complete or in sulphate-deprived medium were used to extract Poly(A)+RNA. The response to sulphate deprivation, which is known to increase the uptake capacity up to ten times, was manifested also by the expression of three mRNA species, as shown by the in vitro translation of the mRNA population. One hour after transfer to complete nutrient medium all three mRNAs were still present.
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Abbreviations
- BSA:
-
Bovine Serum Albumine
- DTT:
-
1,4-Dithio-DL-Threitol
- EDTA:
-
Ethylenediaminetetraacetic-acid
- MES:
-
2-(N-Morpholino) ethane sulfonic acid
- SDS:
-
Sodium Dodecyl Sulfate
- TCA:
-
Trichloracetic acid
- TRIS:
-
Tris(hydroxy-methyl)-aminomethane
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Malagoli, M., Trettenero, A. & Ferrari, G. Response of root mRNA population to sulphate shortage in maize seedlings. Plant Soil 162, 309–313 (1994). https://doi.org/10.1007/BF01347719
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DOI: https://doi.org/10.1007/BF01347719