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The effects of micro-organisms on plant growth

I. Development of roots and root hairs in sand and agar

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Summary

Subterranean clover, tomato, phalaris, and radiata pine were grown with a complete plant- nutrient solution in sterile sand and agar and inoculated with soil suspensions prepared from unsterilized and from sterilized soil.

The presence of micro-organisms reduced primary-root growth in all plants and total root growth in most plants. The total numbers of secondary roots were lower in non-sterile treatments but there was a tendency for an increase in the concentrations of secondary roots with the non-sterile plants. Under the test conditions only radiata pine grown in sterile sand produced significantly greater top growth than those grown in the presence of micro-organisms. Root-stunting micro-organisms were shown to occur in each of four different soil types used in the studies but the extent of stunting varied with the soil. In agar, root stunting was observed at 5 days and 9 days after planting (and inoculation) with subterranean clover and tomato respectively.

Production and growth of root hairs by subterranean clover was markedly reduced by organisms from all four soils tested, the reduction varying with the soil. In contrast to root-stunting organisms, root-hair suppressing micro-organisms were abundant in soil. Root-hair suppression was apparent in sand after 3 days and is an inhibition of root-hair development rather than microbial digestion of existing root hairs. Only slight root-hair reduction was observed for tomato and phalaris.

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References

  1. Åberg, B., On auxin antagonists and synergists in root growth. Physiol. Plantarium3, 447–61 (1950).

    Google Scholar 

  2. Åberg, B., Studies on plant growth regulators. XIII. Some experiments with cress roots. Kgl. Lantbrukshögskol. Ann.23, 479–87 (1957).

    Google Scholar 

  3. Allison, F. E., Azotobacter inoculation of crops: I. Historical. Soil Sci.64, 413–29 (1947).

    Google Scholar 

  4. Askinazi, D. L., The possible role of micro-organisms in increasing the effectiveness of ground rock phosphate as a fertilizer (a review). Soviet Soil Science (Pochvovedeniye)4, 372–79 (1958).

    Google Scholar 

  5. Barnes, R. L. and Naylor, A. W., Effect of various nitrogen sources on growth of isolated roots ofPinus serotina. Physiol. Plantarum12, 82–89 (1959).

    Google Scholar 

  6. Brian, P. W., The ecological significance of antibiotic production.In: “Microbial Ecology” p. 168–188. Seventh Symposium of the Society for General Microbiology. Cambridge University Press (1957).

  7. Bromfield, S. M., The properties of a biologically formed manganese oxide; its availability to oats and its solution by root washings. Plant and Soil9, 325–37 (1958).

    Google Scholar 

  8. Caron, A., Landwirtschaftlich-bakteriologische Probleme. Landwirtsch Vers. Sta.45, 401–18 (1895).

    Google Scholar 

  9. Clark, F. E., Soil micro-organisms and plant roots. Advances Agron.1, 241–88 (1949).

    Google Scholar 

  10. Cooper, R., Bacterial fertilizers in the Soviet Union. Soils and Fertilizers Commonwealth Bur. Soil Sci.22, 327–33 (1959).

    Google Scholar 

  11. Ekdahl, I., Studies on the growth and osmotic conditions of root hairs. Symbolae Botan. Upsaliens.11, 1–86 (1953).

    Google Scholar 

  12. Eliasson, L., Inhibition of the growth of wheat roots in nutrient solutions by substances exuded from the roots. Kgl. Lantbrukshögsk. Ann.25, 285–93 (1959).

    Google Scholar 

  13. Gerretsen, F. C., The influence of micro-organisms on the phosphate intake by the plant. Plant and Soil1, 51–81 (1948).

    Google Scholar 

  14. Gottlieb, D., The mechanism of wilting caused byFusarium bulbigenum var. lycopersici. Phytopathology34, 41–59 (1944).

    Google Scholar 

  15. Hoagland, D. R. and Arnon, D. I., The water culture method of growing plants without soil. Univ. Calif. Agr. Exp. Sta. Circ.347 (1938).

  16. Hiltner, L., Über neuere Erfahrungen und Probleme auf dem Gebiet der Bodenbakteriologie und unter besonderer Berücksichtigung der Gründüngung und Brache. Arb. Deut. Landw. Ger.98, 59–78 (1904).

    Google Scholar 

  17. Jackson, W. T., Effect of indoleacetic acid on rate of elongation of root hairs ofAgrostis alba L. Physiol. Plantarum13, 26–45 (1960).

    Google Scholar 

  18. Jensen, H. L., Bacterial treatment of non-leguminous seeds as an agricultural practice. Australian J. Sci.4, 117–20 (1942).

    Google Scholar 

  19. Katznelson, H., and Bose, B., Metabolic activity and phosphate dissolving capability of bacterial isolates from wheat roots, rhizosphere and non-rhizosphere soil. Can. J. Microbiol.5, 79–85 (1959).

    Google Scholar 

  20. Katznelson, H., Lochhead, A. G., and Timonin, M. I., Soil micro-organisms and the rhizosphere. Botan Rev.14, 543–87 (1948).

    Google Scholar 

  21. Kerr, A., Some interactions between plant roots and pathogenic soil fungi. Australian J. Biol. Sci.9, 45–52 (1956).

    Google Scholar 

  22. Kraskil'nikov, N. A., and Kotelev, V. V., The effect of soil bacteria on the utilization by plants of phosphorus compounds. (In Russian) Dokl. Akad. Nauk.110, 858–61 (1956).

    Google Scholar 

  23. Louw, H. A., and Webley, D. M., A study of soil bacteria dissolving certain mineral phosphate tertilizers and related compounds. J. Appl. Bacteriol.22, 227–33. (1959).

    Google Scholar 

  24. Levisohn, Ida, Forking in pine roots. Nature (London)169, 715 (1952).

    Google Scholar 

  25. Meesters, A., The influence of hetero-auxin on the growth of root hairs and roots ofAgrostemma githago L. — Proc. Kon. Akad. Wetensch. Amsterdam39, 91 (1936).

    Google Scholar 

  26. Norman, A. G., Inhibition of root growth and cation uptake by antibiotics. Soil Sci. Soc. Am. Proc.23, 368–70 (1959).

    Google Scholar 

  27. Nutman, P. S., The influence of the legume in root nodule symbiosis. Biol. Rev.31, 109–51 (1956).

    Google Scholar 

  28. Pantos, G., The main forms of the rhizosphere bacteria of wheat, their physiological properties and their mutual relationship with the plant. (In Hungarian) Kozleményei a Magyar Tudomanyos Akademia Agrártudományok osztályának.9, 315–43 (1956).

    Google Scholar 

  29. Parker, C. A., Non-symbiotic nitrogen fixing bacteria in soil III. Total nitrogen changes in a field soil. J. Soil Sci.8, 48–59 (1957).

    Google Scholar 

  30. Persidsky, D. J., and Wilde, S. A., The effect of volatile substances released by soil humus, and composts on the growth of excised roots. Plant Physiol.29, 474–86 (1954).

    Google Scholar 

  31. Persidsky, D. J., and Wilde, S. A., Effects of eradicants on the microbiological properties of nursery soils. Trans. Wisconsin Acad. Sci.44, 65–73 (1955).

    Google Scholar 

  32. Pikovskaya, R. I., Mineralization of phosphate in soil in connection with the vital activity of certain of micro-organisms. (In Russian) Microbiology (Moscow)17, 362–70 (1948).

    Google Scholar 

  33. Rovira, A. D., Root excretions in relation to the rhizosphere effect IV. Influence of plant species, age of plant, light, temperature, and calcium nutrition on exudation. Plant and Soil11, 53–64 (1959).

    Google Scholar 

  34. Rovira, A. D., and Bowen, G. D., Effect of micro-organisms on the development of roots and root hairs of subterranean clover (T. subterraneum). Nature (London)185, 260–61 (1960).

    Google Scholar 

  35. Sperber, Joan I., The incidence of apatite-solubilizing organisms in the rhizosphere and soil Australian J. Agr. Research6, 778–81 (1958).

    Google Scholar 

  36. Starkey, R. L., Some influences of the development of higher plants upon the micro-organisms in the soil. I. Historical and introductory. Soil Sci.27, 319–34 (1929).

    Google Scholar 

  37. Starkey, R. L., Interrelations between micro-organisms and plant roots in the rhizosphere. Bacteriol. Rev.22, 154–72 (1958).

    Google Scholar 

  38. Stevenson, Greta, Fixation of nitrogen by non-nodulated seed plants. Ann. Bot. N.S.23, 622–35 (1959).

    Google Scholar 

  39. Stolp, H., Beiträge zur Frage der Beziehungen zwischen Mikroorganismen und höheren Pflanzen. Arch. Mikrobiol.17, 1–29 (1952).

    Google Scholar 

  40. Swaby, R. J., Stimulation of plant growth by organic matter. J. Australian Inst. Agr. Sci.8, 156–63 (1942).

    Google Scholar 

  41. Timonin, M. I., Microflora of the rhizosphere in relation to the manganese deficiency disease of oats. Soil Sci. Soc. Am. Proc.11, 284–94 (1946).

    Google Scholar 

  42. Troughton, A., The Underground Organs of Herbage Grasses, 163 pp. Commonwealth Bureau of Pastures and Field Crops Bulletin44, (1957).

  43. Wilson, P. W., The Biochemistry of Symbiotic Nitrogen Fixation, 302 pp. Univ. of Wisconsin Press, U.S.A. (1940).

    Google Scholar 

  44. Wright, Joyce, M., Phytotoxic effects of some antibiotics. Ann. Bot. N.S.15, 493–99 (1951).

    Google Scholar 

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Authors and Affiliations

  1. Division of Soils, C.S.I.R.O., Adelaide, Australia

    G. D. Bowen & A. D. Rovira

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  1. G. D. Bowen
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  2. A. D. Rovira
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Bowen, G.D., Rovira, A.D. The effects of micro-organisms on plant growth. Plant Soil 15, 166–188 (1961). https://doi.org/10.1007/BF01347226

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  • DOI: https://doi.org/10.1007/BF01347226

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