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Microbial populations in surface films and subsurface waters: Amino acid metabolism and growth

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Abstract

Sea surface microlayer (film) and subsurface microbial populations (biomass and activities) were studied in the Damariscotta (Maine) estuary in May and September, 1987. Dissolved free and combined amino acids (DFAA, DCAA), bacterial numbers, microbial ATP, bacterial and microbial DNA synthesis (via3H-thymidine and3H-adenine), and amino acid (3H-glutamic acid) metabolism were measured. DFAA and DCAA were typically enriched in the surface microlayers relative to surface waters, although utilization of glutamic acid was usually more rapid in subsurface waters, as was incorporation of thymidine. Bacteria represented 12 to 40% of the microbial biomass as determined by ATP, except during microalgal blooms in the microlayer. Bacteria were generally not enriched in the surface films, although ATP usually was enriched. Rain input appeared to deplete population densities but stimulated population activities. Two stations which contained similar microbial populations (as estimated by bacterial counts, chlorophylla and ATP) showed very different microbial activities, apparently due to the effects of a substantial rainstorm on one of the stations. The bacterially-dominated processes utilizing thymidine and glutamic acid were enhanced approx five-fold after the rain. Autotrophic carbon production increased approx two-fold, while total microbal community DNA synthesis (as estimated by adenine incorporation into DNA) increased nearly tenfold. The observations of this study indicate that surface microlayers in the temperate waters off the coast of Maine contain highly active heterotrophic and autotrophic populations. The microbial community responds rapidly to changes in nutrient and dissolved organic matter concentrations resulting from both seasonal and temporal effects, including rain and runoff.

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Authors and Affiliations

  1. Scripps Institution of Oceanography, A-018, La Jolla, California, 92093, USA

    A. F. Carlucci & D. M. Wolgast

  2. Skidaway Institute of Oceanography, P.O. Box 13687, 31416, Savannah, Georgia, USA

    D. B. Craven

Authors
  1. A. F. Carlucci
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  2. D. B. Craven
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  3. D. M. Wolgast
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Communicated by M.G. Hadfield, Honolulu

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Carlucci, A.F., Craven, D.B. & Wolgast, D.M. Microbial populations in surface films and subsurface waters: Amino acid metabolism and growth. Mar. Biol. 108, 329–339 (1991). https://doi.org/10.1007/BF01344348

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  • DOI: https://doi.org/10.1007/BF01344348

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