Advertisement

Marine Biology

, Volume 105, Issue 2, pp 191–195 | Cite as

Seasonal variations in the nucleic acid content and RNA:DNA ratio of the gonad of the scallopPecten maximus

  • I. Robbins
  • P. Lubet
  • J. -Y. Besnard
Article

Abstract

The RNA and DNA contents of the male and female portions of the gonad of the hermaphroditic marine bivalve molluscPecten maximus L. have been monitored throughout an annual reproductive cycle, from October 1985–September 1986, for a population from the Baie de Seine (Normandy, France). The DNA content of the male gonad was consistently 5 to 10 times greater than that of the female gonad, whilst the RNA content was lower. This underlines the basic premise that spermatogenesis involves the production of large numbers of small gametes, whereas oogenesis involves the production of few, large, synthetically active gametes. The RNA:DNA ratio is a good indicator of sexual maturation in the male gonad. Two peaks of RNA:DNA ratio were observed for the female gonad, possibly corresponding to gonad restoration and vitellogenesis.

Keywords

Nucleic Acid Seasonal Variation Acid Content Bivalve Reproductive Cycle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. Båmstedt, U., Skjoldal, H. R. (1976). Studies on the deep-water pelagic community of Korsfjorden, Western Norway. Adenosine phosphates and nucleic acid inEuchaeta norvegica (Copepoda) in relation to its life cycle. Sarsia 60: 63–80Google Scholar
  2. Bergeron, J. P., Alayse-Danet, A. M. (1981). Aspartate transcarbamylase de la coquille Saint-JacquesPecten maximus L. (mollusque lamellibranche): méthode de dosage et variation de l'activité dans le manteau et la gonade. J. exp. mar. Biol. Ecol. 50: 99–111Google Scholar
  3. Bergeron, J. P., Buestel, D. (1979). L'aspartate transcarbamylase, indice de l'activité sexuelle de la coquille Saint-Jacques (Pecten maximus L.). Premiers résultats. In: Naylor, E., Hartnoll, R. G. (eds.) Cyclic phenomena in marine plants and animals. Pergamon Press, Oxford, p. 301–308Google Scholar
  4. Besnard, J.-Y. (1988). Etude des constituants lipidiques dans la gonade femelle et les larves dePecten maximus L. (mollusque lamellibranche). Thése doctorale, Université de CaenGoogle Scholar
  5. Bulow, F. J. (1987). RNA-DNA ratios as indicators of growth in fish: a review. In: Summerfelt, R. C. Hall, G. E. (eds.) Age and growth of fish. Iowa State University Press, Iowa, p. 45–64Google Scholar
  6. Bulow, F., J., Zeman, M. E., Winningham, J. R., Hudson, W. F. (1981). Seasonal variations in RNA-DNA ratios and in indicators of feeding, reproduction, energy storage, and condition in a population of bluegill,Lepomis macrochirus Rafinesque. J. Fish Biol. 18: 237–244Google Scholar
  7. Church, R. B., Robertson, F. W. (1966). A biochemical study of the growth ofDrosophila melanogaster. J. exp. Zool. 162: 337–352Google Scholar
  8. Comely, C. A. (1974). Seasonal variations in the flesh weights and biochemical content of the scallopPecten maximus L. in the Clyde Sea area. J. Cons. int. Explor. Mer 35: 281–295Google Scholar
  9. Dagg, M. J., Littlepage, J. L. (1972). Relationships between growth rate and RNA, DNA, protein and dry weight inArtemia salina andEuchaeta elongata. Mar. Biol. 17: 162–170Google Scholar
  10. Kapuscinski, J., Skoczylas, B. (1977). Simple and rapid fluorimetric method for DNA microassay. Analyt. Biochem. 83: 252–257Google Scholar
  11. Kelley, N. R., Ashwood-Smith, M. J., Ellis, D. V. (1982). Duration and timing of spermatogenesis in a stock of the musselMytilus californianus. J. mar. biol. Ass. U.K. 62: 509–519Google Scholar
  12. Lang, C. A., Lau, H. Y., Jefferson, D. J. (1965). Protein and nucleic acid changes during growth and aging in the mosquito. Biochem. J. 95: 372–377Google Scholar
  13. Leick, V. (1968). Ratios between contents of DNA, RNA and protein in different micro-organisms as a function of maximal growth rate. Nature, Lond. 217: 1153–1155Google Scholar
  14. Lone, K. P., Ince, B. W. (1983). Cellular growth responses of rainbow trout (Salmo gairdneri) fed different levels of dietary protein, and an anabolic steroid ethylestrenol. Gen. comp. Endocr. 49: 32–49Google Scholar
  15. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J. (1951). Protein measurements with Folin-phenol reagent. J. biol. Chem. 193: 265–275Google Scholar
  16. Lubet, P. (1959). Recherches sur le cycle sexuel et l'emission des gamètes chez les Mytilidae et les Pectinidae (moll. bivalves). Revue Trav. Inst. (scient. tech.) Pêch. marit. 23: 387–548Google Scholar
  17. Lubet, P. (1986). The reproductive strategy of marine bivalve molluscs. Adv. Invertebrate Reprod. 4: 401–408Google Scholar
  18. Lubet, P., Albertini, L., Robbins, I. (1986). Recherches expérimentales au cours de cycles annuels sur l'action gonadotrope exercée par les ganglions cérébroïdes sur la gamétogenèse femelle chez la mouleMytilus edulis L. (Mollusque bivalve). C. r. hebd. Séanc. Acad. Sci., Paris 303: 575–580Google Scholar
  19. Lubet, P., Besnard, J.-Y., Faveris, R., Robbins, I. (1987). Physiologie de la reproduction de la coquille Saint-Jacques (Pecten maximus L.). Océanis 13: 265–290Google Scholar
  20. Mason, J. (1958). The breeding of the scallopPecten maximus L. in Isle of Man waters. J. mar. biol. Ass. U.K. 36: 473–492Google Scholar
  21. Mathieu, M., Bergeron, J. P., Alayse-Danet, A. M. (1982). L'aspartate transcarbamylase, indice d'activité gametogénétique chez la mouleMytilus edulis L. Int. J. Invertebrate Reprod. (Amsterdam) 5: 337–343Google Scholar
  22. Munro, H. N., Fleck, A. (1966). Recent developments in the measurement of nucleic acids in biological materials. Analyst, Lond. 91: 78–88Google Scholar
  23. Pease, A. K. (1976). Studies of the relationship of RNA/DNA ratios and the rate of protein synthesis to growth in the oyster,Crassostrea virginica. Tech. Rep. Fish. mar. Serv. Can. 622: 1–77Google Scholar
  24. Regnault, M., Luquet, P. (1974). Study by evolution of nucleic acid content of prepuberal growth in the shrimpCrangon vulgaris. Mar. Biol. 25: 291–298Google Scholar
  25. Skjoldal, H. R., Båmstedt, U. (1976). Studies on the deep-water pelagic community of Korsfjorden, Western Norway. Adenosine phosphates and nucleic acids inMeganyctiphanes norvegica (Euphausiacea) in relation to the life cycle. Sarsia 61: 1–14Google Scholar
  26. Sulkin, S. D., Morgan, R. P., II., Minasian, L. L., Jr. (1975). Biochemical changes during larval development of the xanthid crabRhithropanopeus harrisii. II. Nucleic acids. Mar. Biol. 32: 113–117Google Scholar
  27. Sutcliffe, W. H., Jr. (1965). Growth estimates from ribonucleic acid content in some small organisms. Limnol. Oceanogr. 10: 253–258Google Scholar
  28. Sutcliffe, W. H., Jr. (1969). Relationship between growth rate and ribonucleic acid concentration in some invertebrates. J. Fish. Res. Bd Can. 27: 606–609Google Scholar
  29. Thompson, R. J. (1977). Blood chemistry, biochemical composition, and the annual reproductive cycle in the giant scallop,Placopecten magellanicus, from Southeast Newfoundland. J. Fish. Res. Bd Can. 34: 2104–2116Google Scholar
  30. Thompson, R. J. (1984). The reproductive cycle and physiological ecology of the musselMytilus edulis in a subarctic, nonestuarine environment. Mar. Biol. 79: 277–288Google Scholar
  31. Vickers, D. H., Mitlin, N. (1966). Changes in nucleic acid content of the boll weevilAnthonomus grandis Bodeman during its development. Physiol. Zoöl. 39: 70–76Google Scholar
  32. Wright, D. A., Hetzel, E. W. (1985). Use of RNA:DNA ratios as an indicator of nutritional stress in the American oysterCrassostrea virginica. Mar. Ecol. Prog. Ser. 25: 199–206Google Scholar
  33. Wright, D. A., Martin, F. D. (1985). The effect of starvation on RNA-DNA ratios and growth of larval striped bass (Morone saxatalis). J. Fish Biol. 27: 479–486Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • I. Robbins
    • 1
  • P. Lubet
    • 1
  • J. -Y. Besnard
    • 1
  1. 1.Laboratoire de ZoologieUniversité de CaenCaen cédexFrance

Personalised recommendations