Summary
The plant growth regulator 2,4-D inhibits the incorporation of P32O4 into ATP and ADP by Chlorella to a much greater extent than it does the accumulation of inorganic phosphate by the cells.
Comparison of 2,4-D effects on P32 incorporation into nucleotide-P with those of 2,4-dinitrophenol shows that when supplied as equal concentrations of undissociated acid molecules, they are nearly equally effective.
Both 2,4-D and DNP in concentrations which inhibit the incorporation of P32 into nucleotides stimulate the uptake of oxygen by Chlorella, supporting the suggestion that they are effectively uncoupling phosphorylation.
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References
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Wedding, R.T., Black, M.K. Uncoupling of phosphorylation in chlorella by 2,4-dichlorophenoxyacetic acid. Plant Soil 14, 242–248 (1961). https://doi.org/10.1007/BF01343855
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DOI: https://doi.org/10.1007/BF01343855