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Increased radioresistance of cells in cultured multicell spheroids

II. Kinetic and cytogenetic studies

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Summary

Using the Chinese hamster cell line B14 FAF28, several specific properties of the contact effect (CE) of radiation action in spheroids were investigated. CE was found to “protect” the spheroid cells against several types of radiation-induced cytogenetic misfunctions such as blockage in S and G2+M-phase, mutagenesis, and chromosome damage.

However, repair of DNA strand-breaks was the same in monolayers and spheroids. Furthermore, CE is a property of the single cell and does not depend on the proliferative status (cycling or non-cycling) of the cells. We conclude that CE is the result of a physiological modification of the cells occurring during growth in the three-dimensional spheroid matrix and requiring metabolic cooperation and cyclic AMP for its induction.

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Authors and Affiliations

  1. Kernforschungszentrum Karlsruhe, Institut für Genetik und für Toxikologie von Spaltstoffen, Postfach 3640, D-7500, Karlsruhe 1, Federal Republic of Germany

    G. Hinz & H. Dertinger

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  1. G. Hinz
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  2. H. Dertinger
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Hinz, G., Dertinger, H. Increased radioresistance of cells in cultured multicell spheroids. Radiat Environ Biophys 21, 255–264 (1983). https://doi.org/10.1007/BF01341462

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  • DOI: https://doi.org/10.1007/BF01341462

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