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Ultrastructural immuno-localization of pectins and glycoproteins inArabidopsis thaliana pollen grains

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Summary

With the monoclonal antibodies MAC 207 and JIM 8 we could demonstrate the presence and localization of arabino-galactan glycoproteins in pollen grains ofArabidopsis thaliana, with JIM 5 the presence and localization of unesterified pectins. MAC 207 preferentially labels the peripheral plasma membrane of the vegetative cell, whereas JIM 8 specifically labels the area of the sperm cell “wall”. Unesterified pectins are present in the inline layer near the plasma membrane, and in the outer intine region which contains remnants of cytoplasmic channels.

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Authors and Affiliations

  1. Department of Plant Cytology and Morphology, Agricultural University Wageningen, Arboretumlaan 4, NL-6703, BD Wageningen, The Netherlands

    A. C. Van Aelst & J. L. Van Went

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  1. A. C. Van Aelst
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  2. J. L. Van Went
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Van Aelst, A.C., Van Went, J.L. Ultrastructural immuno-localization of pectins and glycoproteins inArabidopsis thaliana pollen grains. Protoplasma 168, 14–19 (1992). https://doi.org/10.1007/BF01332646

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  • DOI: https://doi.org/10.1007/BF01332646

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