Advertisement

Radiation and Environmental Biophysics

, Volume 13, Issue 2, pp 105–113 | Cite as

Photochemically induced cross-links between DNA and alcohol dehydrogenase or salmine, respectively

  • B. Toth
  • K. Dose
Article

Summary

Model experiments with two structurally different proteins (alcohol dehydrogenase and salmine) show that glycine, alanine, and tyrosine are by far more frequently involved in photochemically induced cross-link formations with DNA than is cysteine. The yields for cross-link formation of thymidine with salmine (cysteine-free) are about as high as those with alcohol dehydrogenase (a thiol protein).

Keywords

Alcohol Tyrosine Glycine Cysteine Alanine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alexander, P., Moroson, H. L.: Cross-linking of deoxyribonucleic acid to protein following ultra-violet irradiation of different cells. Nature (Lond.)194, 882–883 (1962)Google Scholar
  2. Boyer, P. D.: Spectrophotometric study of the reaction of protein sulfhydryl groups with organic mercurials. J. Amer. chem. Soc.76, 4331–4337 (1954)Google Scholar
  3. Braun, A., Merrick, B.: Properties of the ultraviolet-light-mediated binding of bovine serum albumin to DNA. Photochem. Photobiol.21, 243–247 (1975)Google Scholar
  4. Ceriotti, G.: A microchemical determination of desoxyribonucleic acid. J. biol. Chem.198, 297–303 (1952)Google Scholar
  5. Collins, M. A., Grant, R. A.: Ultraviolet light induced free radicals in glycine peptides in the solid state. Photochem. Photobiol.9, 369–375 (1969)Google Scholar
  6. Fisher, G. J., Varghese, A. J., Johns, H. E.: Ultraviolet-induced reactions of thymine and uracil in the presence of cysteine. Photochem. Photobiol.20, 109–120 (1974)Google Scholar
  7. Gorelic, L. S., Lisagor, P., Yang, N. C.: The photochemical reactions of 1,3-dimethyluracil with 1-aminopropane and poly-l-lysine. Photochem. Photobiol.16,465–480 (1972)Google Scholar
  8. Hélène, C.: Energy transfer between nuclei acid bases and tryptophan in aggregates and in oligopeptide - nucleic acid complexes. Photochem. Photobiol.18, 255–262 (1973)Google Scholar
  9. Jellinek, T., Johns, R. B.: The mechanism of photochemical addition of cysteine to uracil and formation of dihydrouracil. Photochem. Photobiol.11, 349–359 (1970)Google Scholar
  10. Markovitz, A.: Ultraviolet light-induced stable complexes of DNA and DNA polymerase. Biochim. biophys. Acta (Amst.)281, 522–534 (1972)Google Scholar
  11. Meybeck, A., Meybeck, J.: Photosensitization by phenylalanine of carboxylic acids, amides, and peptides in frozen aqueous solutions. Photochem. Photobiol.16, 359–370 (1972)Google Scholar
  12. Meybeck, A., Windle, J. J.: An E.P.R. study of peptides after U.V. irradiation. Photochem. Photobiol.10, 1–12 (1969)Google Scholar
  13. Risi, S., Dose, K., Rathinasamy, T. K., Augenstein, L.: The effect of environment on cystine disruption by ultraviolet light. Photochem. Photobiol.6, 423–436 (1967)Google Scholar
  14. Salomon, J., Elad, D.: Ultraviolet andγ-ray-induced reactions of nucleic acid constituents. Reactions of purines with amines. Photochem. Photobiol.19, 21–27 (1974)Google Scholar
  15. Smith, K. C.: Dose dependent decrease in extractability of DNA from bacteria following irradiation with ultraviolet light or visible light plus dye. Biochem. biophys. Res. Commun.8, 157–163 (1962)Google Scholar
  16. Smith, K. C.: The photochemical interaction of deoxyribonucleic acid and protein in vivo and its biological importance. Photochem. Photobiol.3, 415–427 (1964)Google Scholar
  17. Smith, K. C.: The biological importance of UV induced DNA-protein cross-linking in vivo and its probable chemical mechanisms. Photochem. Photobiol.7, 651–660 (1968)Google Scholar
  18. Smith, K. C.: Photochemical addition of amino acids to14C-uracil. Biochem. biophys. Res. Commun.34, 354–357 (1969)Google Scholar
  19. Smith, K. C.: A mixed photoproduct of thymine and cysteine: 5-S-Cysteine, 6-Hydrothymine. Biochem. biophys. Res. Commun.39, 1011–1016 (1970)Google Scholar
  20. Smith, K. C., Aplin, R. T.: A mixed photoproduct of uracil and cysteine (5-S-cysteine-6-hydrouracil). A possible model for the in vivo cross-linking of DNA and protein by ultraviolet light. Biochem.5, 2125–2130 (1966)Google Scholar
  21. Smith, K. C., Meun, D. H. C.: Kinetics of the photochemical addition of [35S] cysteine to polynucleotides and nucleic acids. Biochem.7, 1033–1037 (1968)Google Scholar
  22. Smith, K. C., Hodgkins, B., O'Leary, M. E.: The biological importance of ultraviolet light induced DNA-protein cross-links inEscherichia coli 15 TAU. Biochim. biophys. Acta (Amst.) 114, 1–15 (1966)Google Scholar
  23. Varghese, A. J.: Photochemical addition of glutathione to uracil and thymine. Photochem. Photobiol.20, 339–343 (1974a)Google Scholar
  24. Varghese, A. J.: Photoreactions of 5-bromouracil in the presence of cysteine and glutathione. Photochem. Photobiol.20, 461–464 (1974b)Google Scholar
  25. Wheeler, O. H., Julián, D. A., Ribot, R. A.: Photolysis and radiolysis of phenylalanylglycine. Photochem. Photobiol.12, 505–508 (1970)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • B. Toth
    • 1
  • K. Dose
    • 1
  1. 1.Institut für BiochemieJohannes Gutenberg-UniversitätMainzFederal Republic of Germany

Personalised recommendations